Hidden interactions in the intertidal rocky shore: variation in pedal mucus microbiota among marine grazers that feed on epilithic biofilm communities

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Microbiology

Main article text

 

Introduction

Materials & Methods

Grazer assemblage

Pedal mucus microbiota analyses

DNA extraction and 16S rRNA-gene sequencing

Community analyses

Pedal mucus protein and carbohydrate concentration

Pedal mucus effects on integrated measures of epilithic biofilms abundance

Results

Microbiota and epilithic biofilm microbial communities

Pedal mucus protein and carbohydrate concentration

Pedal mucus effects on epilithic biofilms

Discussion

Microbiota and epilithic biofilm microbial communities

Pedal mucus protein and carbohydrate concentration

Pedal mucus effects on epilithic biofilms

Conclusions

Supplemental Information

Supplementary Information

DOI: 10.7717/peerj.13642/supp-1

Epilithic Biofilm 1 Forward sequence

DOI: 10.7717/peerj.13642/supp-2

Epilithic Biofilm 1 Reverse sequence

DOI: 10.7717/peerj.13642/supp-3

Epilithic Biofilm 5 Forward sequence

DOI: 10.7717/peerj.13642/supp-4

Epilithic Biofilm 5 Reverse sequence

DOI: 10.7717/peerj.13642/supp-5

Epilithic Biofilm 27 Forward sequence

DOI: 10.7717/peerj.13642/supp-6

Epilithic Biofilm 27 Reverse sequence

DOI: 10.7717/peerj.13642/supp-7

Epilithic Biofilm 29 Forward sequence

DOI: 10.7717/peerj.13642/supp-8

Epilithic Biofilm 29 Reverse sequence

DOI: 10.7717/peerj.13642/supp-9

C. granosus 47 Forward sequence

DOI: 10.7717/peerj.13642/supp-10

C. granosus 47 Reverse sequence

DOI: 10.7717/peerj.13642/supp-11

C. granosus 48 Forward sequence

DOI: 10.7717/peerj.13642/supp-12

C. granosus 48 Reverse sequence

DOI: 10.7717/peerj.13642/supp-13

C. granosus 49 Forward sequence

DOI: 10.7717/peerj.13642/supp-14

C. granosus 49 Reverse sequence

DOI: 10.7717/peerj.13642/supp-15

C. granosus 50 Forward sequence

DOI: 10.7717/peerj.13642/supp-16

C. granosus 50 Reverse sequence

DOI: 10.7717/peerj.13642/supp-17

E. peruviana 57 Forward sequence

DOI: 10.7717/peerj.13642/supp-18

E. peruviana 57 Reverse sequence

DOI: 10.7717/peerj.13642/supp-19

E. peruviana 58 Forward sequence

DOI: 10.7717/peerj.13642/supp-20

E. peruviana 58 Reverse sequence

DOI: 10.7717/peerj.13642/supp-21

E. peruviana 59 Forward sequence

DOI: 10.7717/peerj.13642/supp-22

E. peruviana 58 Reverse sequence

DOI: 10.7717/peerj.13642/supp-23

E. peruviana 60 Forward sequence

DOI: 10.7717/peerj.13642/supp-24

E. peruviana 60 Reverse sequence

DOI: 10.7717/peerj.13642/supp-25

F. crassa 38 Forward sequence

DOI: 10.7717/peerj.13642/supp-26

F. crassa 38 Reverse sequence

DOI: 10.7717/peerj.13642/supp-27

F. crassa 40 Forward sequence

DOI: 10.7717/peerj.13642/supp-28

F. crassa 40 Reverse sequence

DOI: 10.7717/peerj.13642/supp-29

F. crassa 53 Forward sequence

DOI: 10.7717/peerj.13642/supp-30

F. crassa 53 Reverse sequence

DOI: 10.7717/peerj.13642/supp-31

F. crassa 54 Forward sequence

DOI: 10.7717/peerj.13642/supp-32

F. crassa 54 Reverse sequence

DOI: 10.7717/peerj.13642/supp-33

F. crassa 55 Forward sequence

DOI: 10.7717/peerj.13642/supp-34

F. crassa 55 Reverse sequence

DOI: 10.7717/peerj.13642/supp-35

S. araucana 41 Forward sequence

DOI: 10.7717/peerj.13642/supp-36

S. araucana 41 Reverse sequence

DOI: 10.7717/peerj.13642/supp-37

S. araucana 42 Forward sequence

DOI: 10.7717/peerj.13642/supp-38

S. araucana 42 Reverse sequence

DOI: 10.7717/peerj.13642/supp-39

S. araucana 43 Forward sequence

DOI: 10.7717/peerj.13642/supp-40

S. araucana 43 Reverse sequence

DOI: 10.7717/peerj.13642/supp-41

S. araucana 44 Forward sequence

DOI: 10.7717/peerj.13642/supp-42

S. araucana 44 Reverse sequence

DOI: 10.7717/peerj.13642/supp-43

S. araucana 45 Forward sequence

DOI: 10.7717/peerj.13642/supp-44

S. araucana 45 Reverse sequence

DOI: 10.7717/peerj.13642/supp-45

S. lessoni 62 Forward sequence

DOI: 10.7717/peerj.13642/supp-46

S. lessoni 62 Reverse sequence

DOI: 10.7717/peerj.13642/supp-47

S. lessoni 63 Forward sequence

DOI: 10.7717/peerj.13642/supp-48

S. lessoni 63 Reverse sequence

DOI: 10.7717/peerj.13642/supp-49

S. lessoni 64 Forward sequence

DOI: 10.7717/peerj.13642/supp-50

S. lessoni 64 Reverse sequence

DOI: 10.7717/peerj.13642/supp-51

S. lessoni 65 Forward sequence

DOI: 10.7717/peerj.13642/supp-52

S. lessoni 65 Reverse sequence

DOI: 10.7717/peerj.13642/supp-53

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Clara Arboleda-Baena conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Claudia Belén Pareja performed the experiments, prepared figures and/or tables, and approved the final draft.

Isadora Pla performed the experiments, prepared figures and/or tables, and approved the final draft.

Ramiro Logares analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Rodrigo De la Iglesia conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Sergio Andrés Navarrete conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

Comité Institucional de Seguridad en Investigación - Pontificia Universidad Católica de Chile approved the study (170829006).

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The sequence data are available at the European Nucleotide Archive (ENA) database: PRJEB41739.

Data Availability

The following information was supplied regarding data availability:

The data is available at Figshare: https://figshare.com/projects/Hidden_interactions_in_the_intertidal_rocky_shore_variation_in_pedal_mucus_microbiomes_among_marine_grazers_that_feed_on_epilithic_biofilm_communities_/119655

Arboleda Baena, Clara María (2021): PedalMucusEffect_PhotosyntheticBiofilmCoverPercentage. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15170586.v1

Arboleda Baena, Clara María (2021): PedalMucus_MANOVA_Carbohydrates/ProteinsContent. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15170523.v1

Arboleda Baena, Clara María (2021): PedalMucus_ProteinsContent. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15170520.v1

Arboleda Baena, Clara María (2021): PedalMucus_CarbohydratesContent. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15170514.v1.

Arboleda Baena, Clara María (2021): GrazerAssemblageBiomass.csv. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15113490.v2.

Arboleda Baena, Clara María (2021): PedalMucus_pH. figshare. Dataset. https://doi.org/10.6084/m9.figshare.15115059.v2.

Funding

Funding for these studies and for international collaboration was provided by CONICYT–National PhD scholarship Program 2016 and CONICYT (Chilean National Agency for Research and Development (ANID)) international complement to CMAB, and by Fondecyt grants No. 1160289 and 1200636 to Sergio Navarrete, and No. 1171259 to Rodrigo De la Iglesia. Complementary funding was provided by Chilean National Agency for Research and Development (ANID) PIA/BASAL FB0002 to Sergio Navarrete. The small grants for undergraduate research involvement, for winter 2018 and summer 2019, was provided by the Pontificia Universidad Católica de Chile. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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