DNA barcoding and morphological identification of spiny lobsters in South Korean waters: a new record of Panulirus longipes and Panulirus homarus homarus

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Hettiarachchi et al. present #DNA barcoding and morphological identification of spiny #lobsters in South Korean waters: a new record of Panulirus longipes and Panulirus homarus homarus Read the full article https://t.co/gWVyBhYLJ0 #Aquaculture #Fisheries https://t.co/AgBBUM2SDU
Hettiarachchi et al. present #DNA barcoding and morphological identification of spiny #lobsters in South Korean waters: a new record of Panulirus longipes and Panulirus homarus homarus Read the full article https://t.co/KKFzDOmUPy #Aquaculture #Fisheries https://t.co/ELfBYc1Uu9
Aquatic Biology

Main article text

 

Introduction

Materials & Methods

Sample collection

Examination of morphological features

DNA Extraction and PCR amplification

COI cloning and sequencing

Alignment and phylogenetic analyses

Results

Phylogenetic analyses of spiny lobster COI genes

Morphological examination

Discussion

Conclusions

Supplemental Information

Photographs of dorsal and ventral sides, lateral side of the carapace and dorsal side of the abdomen somites of P. japonicus (Holthuis morphotype) collected from Jeju Island, South Korea

A) Dorsal side, B) Ventral side; C) Lateral side of the carapace; fh: Dark greenish brown frontal horns with white spots and orange color ventral margin; s: Randomly scattered spines on carapace with black color basal area; lm: White spotted lateral margin of the carapace; Enlarged area demarcated by rectangle: Reddish brown hairs on the mid-dorsal area of the carapace. D) Dorsal side of the abdomeinal somites; a: Non-interrupted transverse groove with posteriorly directed hairs; b: Posterior margin of the second somite with posteriorly directed hairs; c: Brown to purplish color base of telson; d: Reddish brown and slightly curved posterior margin of telson.

DOI: 10.7717/peerj.12744/supp-1

Photograph of lateral side of the abdominal somites of P. japonicus (Holthuis morphotype) collected from Jeju Island, South Korea and it’s diagrammatic view

Arrows with a lowercase letter of photograph are indicating the following morphological features respectively; a: Posteriorly directed hairs in transverse groove; b: Posteriorly directed hairs in posterior margin of the second somite; c: Curved transverse groove at the lateral end of second, third and fourth somites; d: Interconnection between transverse groove and pleural groove at first somite; e: Gap between transverse groove and pleural groove at third somite; f: Gap between transverse groove and pleural groove at second somite. Scale bar represents two cm.

DOI: 10.7717/peerj.12744/supp-2

NCBI GenBank accession numbers obtained for COI sequences and sampling sites of spiny lobsters collected for this study

DOI: 10.7717/peerj.12744/supp-3

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Sachithra Amarin Hettiarachchi conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, all the drawings were done, and approved the final draft.

Ji-Yeon Hyeon and Angka Mahardini conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Hyung-Suk Kim and Han-Jun Kim performed the experiments, authored or reviewed drafts of the paper, collected spiny lobsters by Scuba diving, and approved the final draft.

Jun-Hwan Byun performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Jong-Gyun Jeong, Jung-Kyu Yeo and Shin-Kwon Kim performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Se-Jae Kim, Youn-Seong Heo and Do-Hyung Kang conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Jonathan Sathyadith analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Sung-Pyo Hur conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, collected spiny lobsters by Scuba diving, and approved the final draft.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

The Animal care and use committee of the Jeju National University approved the study (2020-0012).

Data Availability

The following information was supplied regarding data availability:

The COI gene sequences are available at GenBank: MZ203547 to MZ203550 and OK037046 to OK037058.

Funding

This research was funded by the National Institute of Fisheries Science, South Korea (R2021012) and Korea Institute of Ocean Science and Technology (PE99922). The funders had no roles in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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