Roles of H2S and NO in regulating the antioxidant system of Vibrio alginolyticus under norfloxacin stress

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Microbiology

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Introduction

Materials and Methods

Bacteria strain, culture media and harvest

Primers and reagents

Preparation for stock solutions

Determination of the minimum inhibitory concentration (MIC) of NOR against V. alginolyticus

Sample treatment with NOR

Measurement of the total protein content

Interaction of H2S and NO

Measurement of CBS and NOS contents

Measurement of H2S and NO contents

Measurement of H2O2

Measurement of the relative expression level of antioxidant-related genes

Statistical analysis

Results

Minimum inhibitory concentration (MIC) of NOR

Effect of NOR treatment on V. alginolyticus

Effect of NO on CBS and H2S content in V. alginolyticus

Effect of H2S on NOS and NO content in V. alginolyticus

H2O2 accumulation and relative expression level of antioxidant-related genes under donors or scavengers

Discussion

Conclusion

Supplemental Information

The raw datasets used and/or analyzed for the current study

DOI: 10.7717/peerj.12255/supp-1

Open reading frame and deduced amino acid sequences of SOD,CAT,GR gene in Vibrio alginolyticus HY9901

DOI: 10.7717/peerj.12255/supp-2

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Shuhe Chen and Yu Ding conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Yunsheng Chang conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The raw measurements and DNA sequence used in designing the primers are provided in the Supplementary Files.

Funding

This work was supported by the grants from the Natural Science Foundation of Guangdong Province (No. 2014A030313604), the Graduate Education Innovation Program of Guangdong Ocean University (No. 201724) and the Foundation for the Distinguished Young Talents in Higher Education of Guangdong, China. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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