Anti-inflammatory and antiaging properties of chlorogenic acid on UV-induced fibroblast cell

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Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials and Methods

Materials

Measurement of IL-1β and TNF-α

Total protein measurements

Measurement of ROS level

Measurement of apoptotic, live, necrotic cells

Measurement of COL-3 gene expression

Statistical analysis

Results

IL-1β level

TNF-α level

ROS level

Apoptosis level

COL-3 gene expression

Discussion

Conclusion

Supplemental Information

Apoptosis Level Data.

DOI: 10.7717/peerj.11419/supp-1

COL-3 Gene Expression Data.

DOI: 10.7717/peerj.11419/supp-2

IL- 1β Level Data.

DOI: 10.7717/peerj.11419/supp-3

TNF-α Level Data.

DOI: 10.7717/peerj.11419/supp-5

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Ermi Girsang conceived and designed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Chrismis N. Ginting conceived and designed the experiments, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

I. Nyoman Ehrich Lister conceived and designed the experiments, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Kamila Yashfa Gunawan performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Wahyu Widowati performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The Gen COL-3 and B-Actin sequences are available at NCBI:

COL-3, NM_000090.4; B-Actin, NM_001101.5.

Data Availability

The following information was supplied regarding data availability:

The raw measurements are available in the Supplemental Files.

Funding

This study was supported by The Ministry of Education, Culture, Research, and Technology, Indonesia for research grant of PDUPT (Penelitian Dasar Unggulan Perguruan Tinggi) 2021. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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