Description of three new species of Geometridae (Lepidoptera) using species delimitation in an integrative taxonomy approach for a cryptic species complex

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Biodiversity and Conservation

Main article text

 

Introduction

Materials and Methods

Sampling

Rearing methods

DNA extraction and PCR conditions

Alignment, tree inference and species delimitation

Morphological study

Results

Species Description

Discussion

Supplemental Information

Fasta File with sequences used in this study.

DOI: 10.7717/peerj.11304/supp-1

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Simeão S. Moraes conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Ygor Z. Montebello conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Mariana A. Stanton conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Lydia Fumiko Yamaguchi performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Massuo J. Kato performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

André V.L. Freitas conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

Field permits were granted by ICMBio (Instituto Chico Mendes para Biodiversidade) (permit number 10362-1, permit number 15780-10 and permit number 22205-6).

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The new generated sequences are available at GenBank and in the Supplementary File. The accessions from sequences retrieved from Genbank are also available in Table 1:

GQ433544.1, GQ433557.1, GQ433564.1, GQ433565, GQ433565.1, HQ576249.1, JF859258.1, JQ179870.1, JQ179873.1, JQ179875.1, JQ179880.1, JQ179891.1, JQ179895.1, JQ179901.1, JQ179902.1, JQ179903.1, JQ179905.1, JQ179906.1, JQ179915.1, JQ179923.1, JQ179924.1, JQ179941.1, JQ424371.1, JQ424372.1, JQ424374.1, JQ424375.1, JQ424379.1, JX150863.1, JX150931.1, KU381055.1, KU381316.1, KU381753.1, MG572804.1, MG572805.1, MG572814.1.

Data Availability

The following information was supplied regarding data availability:

The raw data are available in the Supplementary File.

The holotypes and paratypes of new described species are deposited at Zoological Collection of the Museu da Diversidade Biológica da Universidade Estadual de Campinas (ZUEC), Campinas, São Paulo, Brazil (accession numbers LEPSM901, K2452-5, K2452-2, M155-12, M155-3, LEPSM922) and at Museu de Zoologia da Universidade de São Paulo (MZSP), São Paulo, São Paulo, Brazil (accession numbers K2452-1, K2452-4, M157-3, M156-5).

New Species Registration

The following information was supplied regarding the registration of a newly described species:

Publication LSID: urn:lsid:zoobank.org:pub:9450BCDC-7EB9-4CA6-BC76-04324F81ACA4

Eois ewa Moraes & Stanton LSID: urn:lsid:zoobank.org:act:A0C8118B-29C7-41E1-8434-5FFDEBFB3064,

Eois oxum Moraes & Freitas LSID: urn:lsid:zoobank.org:act:CB95F8D8-E007-42D5-8C76-3A3FEE15D967.

Eois oya Moraes & Montebello LSID: urn:lsid:zoobank.org:act:C17CD8C7-D933-4C9E-8307-F74CC05D6CA1.

Funding

This work was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) with the scholarship awarded by SSM (grants FAPESP 2015/17047–5, FAPESP 2016/20196–5), YZM (2019/02727-1) and MAS (FAPESP 2015/26823-9). AVLF thanks the Brazilian Research Council—CNPq (grant 303834/2015-3), the FAPESP (grants no. 2011/50225-3, 2012/50260-6, 2013/50297-0) and The United States Agency for International Development—USAID/the U.S. National Academy of Sciences (NAS), under the PEER program (Sponsor Grant Award Number: AID-OAA-A-11-00012). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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