Lactobacillus reuteri TSR332 and Lactobacillus fermentum TSF331 stabilize serum uric acid levels and prevent hyperuricemia in rats

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Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials & Methods

Probiotic cultivation and analysis of purine nucleoside assimilation in vitro

HPLC

Animal treatment

Hyperuricemia induction and experimental setup

Evaluation of body weight, creatinine, and UA

Statistical analysis

Results

L. reuteri TSR332 and L. fermentum TSF331 rapidly metabolize purine nucleosides, without the production of UAs

L. reuteri TSR332 and L. fermentum TSF331 stabilize the level of UA in hyperuricemia model rats

L. reuteri TSR332 displays preventive and therapeutic effects in stabilizing the serum UA level in hyperuricemia model rats

L. reuteri TSR332 stabilizes the UA level in acute hyperuricemia rat model

Discussion

Conclusions

Supplemental Information

Raw data of Figs. 1, 2, 3 and 5

(1) Preliminary tests of TSF331, TSR332, and La322 in hyperuricemic rats. (2) Pretreatment and treatment effects of TSR332 in hyperuricemic rats. (3) UA, body weight, and kidney function in severe hyperuricemic rats. (5) In vitro purine assimilation assays of inosine and guanosine in probiotic strains.

DOI: 10.7717/peerj.11209/supp-1

Additional Information and Declarations

Competing Interests

Yi-Wei Kuo, Shih-Hung Hsieh, Jui-Fen Chen, Cheng-Ruei Liu, Ching-Wei Chen, Yu-Fen Huang, and Hsieh-Hsun Ho are employed by Glac Biotech Co., Ltd.

Author Contributions

Yi-Wei Kuo, Cheng-Ruei Liu, Ching-Wei Chen and Yu-Fen Huang performed performed the experiments, prepared figures and/or tables, and approved the final draft.

Shih-Hung Hsieh analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Jui-Fen Chen conceived and designed the experiments, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Hsieh-Hsun Ho conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The Ethical Committee of Hungkuang University approved this research (HK-P-10512).

Data Availability

The following information was supplied regarding data availability:

The raw data for Figs. 13 and 5 are available in the Supplementary Tables.

Funding

The authors received no funding for this work.

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