The combination of lactoferrin and linolenic acid inhibits colorectal tumor growth through activating AMPK/JNK-related apoptosis pathway

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Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials and Methods

Chemicals

Cell culture and viability detection

Cell apoptosis detection

Cell invasion detection

Cell scratch analysis

Animal model construction

Metabonomics detection of HT29 cells

Western blotting detection

Statistical analysis

Results

Dosage selections of lactoferrin and three unsaturated fatty acids

Lactoferrin and three unsaturated fatty acids induced apoptosis of HT29 cells

Lactoferrin and three unsaturated fatty acids inhibited invasion and migration of HT29 cells

Lactoferrin and linolenic acid inhibited tumor growth in a tumor bearing nude mouse model

Screening of specific metabolites in HT29 cells treated with lactoferrin, linolenic acid and (lactoferrin + linolenic acid) combination

Lactoferrin + linolenic acid combination activates AMPK/JNK-related apoptosis pathway

Discussion

Conclusions

Supplemental Information

Seven special metabolites screened by metabonomics detection.

DOI: 10.7717/peerj.11072/supp-1

Metabonomics raw data.

DOI: 10.7717/peerj.11072/supp-2

Chemical information.

DOI: 10.7717/peerj.11072/supp-3

Raw data of Western blot in Figure 7.

DOI: 10.7717/peerj.11072/supp-4

Figure raw data.

DOI: 10.7717/peerj.11072/supp-5

Protocol of Metabonomics detection of HT29 cells.

DOI: 10.7717/peerj.11072/supp-6

HT29 cells metabonomics data.

DOI: 10.7717/peerj.11072/supp-7

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Qianqian Yao performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Huiying Li conceived and designed the experiments, performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Linlin Fan performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Shengnan Huang analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Jiaqi Wang conceived and designed the experiments, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Nan Zheng conceived and designed the experiments, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The ethics Committee of the Chinese Academy of Agriculture Sciences (Beijing, China) approved the study (Permission number: CAS20190410, 10th April, 2019).

Data Availability

The following information was supplied regarding data availability:

Raw data are available in the Supplemental Files.

Funding

The present work was supported by the Scientific Research Project for Major Achievements of Agricultural Science and Technology Innovation Program (CAAS-ZDXT2019004), the Ministry of Modern Agro-Industry Technology Research System of China (CARS-36), and the Agricultural Science and Technology Innovation Program (ASTIP-IAS12). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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