Molecular barcode and morphological analysis of Smilax purhampuy Ruiz, Ecuador

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Plant Biology

Main article text

 

Introduction

Materials and Methods

Study area

Collection of plant material

Plant material preparation

Micromorphological analysis

DNA extraction and PCR

Bioinformatic analysis

Results

Morphological analysis

Molecular barcodes for Smilax purhampuy Ruiz plants

Discussion

Microscopic analysis

Molecular barcode sequences

Conclusions

Supplemental Information

Phylogenetic analysis of molecular barcode sequences

DOI: 10.7717/peerj.11028/supp-1

Primers used for amplification of the barcodes psbA-trnH spacer, psbK-psbI spacer, rpoB, rpoC1, atpF-atpH spacer, rbc L, mat K, and ITS2

DOI: 10.7717/peerj.11028/supp-2

Barcode DNA sequences of Smilax purhampuy Ruiz

DOI: 10.7717/peerj.11028/supp-3

Accession numbers of Smilax purhampuy Ruiz (GUAY 13117) barcode sequences at GenBank

DOI: 10.7717/peerj.11028/supp-4

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Pilar Soledispa conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Efrén Santos-Ordóñez conceived and designed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Migdalia Miranda conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Ricardo Pacheco performed the experiments, analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Yamilet Irene Gutiérrez Gaiten performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Ramón Scull performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

Samples were collected for identification in the framework of the GUAY Herbarium, Facultad de Ciencias Naturales, Universidad de Guayaquil.

Data Availability

The following information was supplied regarding data availability:

Sequences are available in the Supplemental Files and at GenBank: MT740231, MT740232, MT740233, MT740234, MT740235, MT740236, MT740237, MT740238, MT740239, MT740240, MT740241, MT740242, MT734663, MT734664, MW300280, MW300281, MW300282, MW300277, MW300278, MW300279.

Funding

The authors received no funding for this work.

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