Developing mathematical model for diurnal dynamics of photosynthesis in Saccharum officinarum responsive to different irrigation and silicon application

Introduction

Water scarcity is one of the most crucial abiotic stresses for plants in the dynamic era of climate change. A number of studies have mentioned that water deficit is more harmful than other abiotic stresses (Chen et al., 2017; Verma et al., 2019a; Li et al., 2019; Raza et al., 2019; Verma et al., 2020). Under limited or highly variable water, plants have developed various mechanisms of resistance to water deficit by reduction of the plant life cycle (Mitra, 2001; Cia et al., 2012). Therefore, plants have evolved in various cellular and molecular strategies to cope with water deficit (Ijaz et al., 2017; Ali et al., 2018). Abiotic stresses are estimated to reduce about 51–82% agricultural crop production. The metabolic changes to water deficit in varieties with various responses to water stress have been well documented in different agricultural crops (Kang et al., 2011; Witt et al., 2012; Silvente, Sobolev & Lara, 2012; Zhao et al., 2014; Verma et al., 2019a, 2020).

Low soil water capacity in the dry season is one of the most important limitations to photosynthesis and consequently to Saccharum officinarum production (Chen et al., 2011; Verma et al., 2019a). Under limited water conditions, disturbances in photosynthetic apparatus at the molecular and cellular levels are associated with low electron transport through photosystem II (PS II) and/or with structural damages of PS II and the light harvesting complexes (Hura et al., 2007; Wu et al., 2008). Photosynthetic and growth responses are dependent on environmental variables and developmental phages. It is expected that changes in temperature, light intensity and available water content, and changes across the growth phases will influence the growth and physiological dynamics during diurnal cycle (De Souza et al., 2018; Verma et al., 2019a). Improving photosynthetic capacity is linked for the enhancement of biomass and crop productivity. Soil water content and nutrients can also play significant roles in sustaining the photosynthetic responses in agricultural crops (Shangguan, Shao & Dyckmans, 2000; Li et al., 2016, 2019; Khan et al., 2017; Verma et al., 2020). Under water deficiency, Si application can improve and/or enhance photosynthetic capacity, root growth-development, nutrient uptake and consequently increase crop productivity (Verma et al., 2019a, 2019b, 2019c; Li et al., 2019). Various agronomic strategies have been adopted for this purpose. One of the advanced strategies is the use of plant bio-stimulator to enhance the adaptability and protection of crop plants subjected to environmental stresses.

Silicon (Si) is the second most important element in the earth’s crust, which can improve crop resistance to reduce the negative impacts of biotic and abiotic stresses such as insufficient water, extreme air temperature, UV, cold, alkalinity, nutritional imbalance, heavy metal toxicity, plant pathogens and insect pests in various crop plants (Liang et al., 2007; Guo et al., 2016; Reynolds et al., 2016; Ju et al., 2017; Chen et al., 2018; Verma et al., 2019a). Plants generally take up Si in the form of silicic acid from soil and nutrient solutions and Si is the only nutrient element that is not detrimental when absorbing excess in the plant’s organ (Ma, Miyake & Takahashi, 2001; Ma & Yamaji, 2006; Chen et al., 2018). The maximum solubility of Si(OH)₄ in solution is nearly 2 mM, and its concentration in soil solutions usually differ between 0.1 and 0.6 mM (Raven, 1983; Epstein, 1994). Moreover, orthosilicic acid (pKa₁ = 9.84, pKa₂ = 13.2, at 25 °C), the form of Si accessible to plants (Casey et al., 2004), is soluble in water only up to about 2 mM at 25 °C, above which polymerization into silica (SiO₂) gels begins to occur (Ma, Miyake & Takahashi, 2001). Under similar situations, plant varieties have different abilities to accumulate Si, a reality that has been known, if poorly understood, for a long time.

The protective role of Si in metabolic, physiological and/or anatomical activities in crop plants against environmental stresses have been widely documented (Van Bockhaven, De Vleesschauwer & Höfte, 2013; Zhu & Gong, 2014; Shi et al., 2016; Verma et al., 2019a). The beneficial effects of Si against limited water supply/water deficiency have been extensively assessed in many crop plants, like Oryza sativa (Ming et al., 2012), Zea mays (Kaya, Tuna & Higgs, 2006; Amin et al., 2014), Triticum aestivum (Pei et al., 2010; Gong & Chen, 2012), Sorghum bicolor (Liu et al., 2014), Solanum lycopersicum (Shi et al., 2016), Saccharum spp. (Verma et al., 2019a, 2019b, 2019c, 2020), cucumber (Ma et al., 2004), Kentucky bluegrass (Saud et al., 2014), canola (Habibi, 2014) and alfalfa (Liu & Guo, 2013).

Sugarcane (Saccharum officinarum L.) is one of the most important cash crop in the globe due to its great demand for sugar and renewable energy sources to replace fossil fuels. Unlikely, in various regions, especially in the tropical and sub-tropical areas, the production of Saccharum officinarum is markedly decreased up to 60% due to availability of insufficient water for irrigation (Robertson et al., 1999; Verma et al., 2019a).

However, knowledge about how Si modulates the morphological, physiological and biomass accumulation in Saccharum officinarum “GT 42” during water stress remains elusive. Although the essentiality of this element to plants is still debated, there have been significant impacts in our understanding of the uptake of Si in plants. In addition, the present database regarding the precise amount of Si for its application method in Saccharum officinarum plants is limited. Therefore, the present study was conducted to investigate the possible impacts of exogenous application of Si on growth, biomass accumulation and photosynthetic responses in Saccharum officinarum plants subjected to water stress. Our work in Saccharum officinarum may help to better understand the mechanisms and functions for Si-mediated water stress tolerance in plants.

Materials and Methods

Saccharum officinarum “GT 42” single bud cane setts were planted in fertile farmland soil in greenhouse in March 2019 at Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, China. After germination (8 weeks), the seedlings were shifted in pots (soil capacity 3.5 kg), and continued to receive full irrigation to keep moisture capacity at 100–95% for proper root development before treatment. For the stressed treatment, limited water was imposed by gradually withdrawing irrigation until 80–75%, 55–50% and 35–30% of soil moisture capacity, determined by Soil Moisture Meter (Top Instrument Co. Ltd., Zhejiang, China). At the end of July, silicon fertilizer was applied as 0, 100, 300 and 500 mg L⁻¹, respectively, directly in the soil. Calcium metasilicate powder (Wollastonite, CaO.SiO₂) was used as a source of Si. The irrigation water was applied to the plant roots to keep 100–95% (normal), 80–75% (mild), 55–50% (moderate) and 35–30% (severe) of soil moisture capacity, respectively. The total amount of Si solution applied to well water and stressed-plants was the same. The climatic variables (ambient air temperature (Ta), air relative humidity (RH), ambient CO₂ concentration (Ca), photosynthetic photon flux density (PPFD) and vapor pressure deficit (VPD)) were recorded diurnally (Fig. 1). The experiment was designed as completely randomized with ten biological replicates.

Results

Effect of silicon on growth and biomass traits

The Saccharum officinarum plants showed a drastic decline in plant height (PH), leaf area-expansion (LAE), leaf relative water content (LWC) and plant biomass (fresh and dry) under water stress, compared to well irrigation control (Figs. 2A–2E). The negative effects of low soil moisture capacity on growth and biomass were significantly (P < 0.05) mitigated and gradually enhanced with increasing Si levels (100–500 mg L⁻¹). PH, LAE, RWC, fresh and dry mass of the plants in 100–95%, 80–75%, 55–50% and 35–30% of soil moisture capacity were found higher than the control without Si, with 2.41–20.48%, 0.65–6.12%, 0.17–9.54%, 0.14–2.42% and 0.30–13.12% increase, respectively (Figs. 2A–2E).

Growth and biomass traits exhibited an initial increase with the application of Si (100–300 mg L⁻¹) and then declined considerably at excess (500 mg L⁻¹). Shoot fresh and dry biomass accumulation in Si-treated plants showed significant (P < 0.05) improvement compared to normal plants under stress condition. The 300 mg L⁻¹ Si concentration exhibited the most significant effects on growth and biomass of Saccharum officinarum plants, followed by 500 mg L⁻¹ Si (Fig. 2).

Water limitation and silicon effects on the diurnal changes of leaf gas exchange

One of the prime impacts of water stress is on the physiological process of photosynthetic responses. Water stress caused a severe loss in photosynthesis. Diurnal changes of photosynthesis are shown in Fig. 3. Overall the pattern of P_N mirrored that of gs. P_N and gs maximum reached at 9:00 as PPFD and gs enhanced. Subsequently, P_N steadily declined in all stressed plants and Si supplemented until a minimum value was reached in the early evening (17:00), similar to gs and E. Hence, gs was the main limiting factor for P_N of mesophyll cells at this time of the day. At 17:00, photosynthetic values were found lowest due to low PPFD and gs. Water and fertilization did affect the trend in the diurnal variation of P_N. Under different soil moisture levels such as 100–95, 80–75, 55–50 and 35–30%, the P_N in the 300 mg L⁻¹ treatment was higher than that of 500 mg L⁻¹ and control (Figs. 3A–3P). The exogenous application of Si effects under non-limiting water and stressed conditions were significantly (P < 0.05) different at each measurement points (Table 1).

Table 1:

Statistical analysis variance of four silicon concentrations on photosynthetic CO₂ assimilation rate (P_N), stomatal conductance (gs) and leaf transpirational rate (E) of Saccharum officinarum “GT 42” plants at diurnal and different soil water availabilities.

Treatment (%FC)	Time (h)
	07:00	09:00	11:00	13:00	15:00	17:00
Effect of Si on PN
100–95	NS	**	**	**	NS	NS
80–75	**	**	**	**	**	NS
55–50	**	NS	NS	NS	NS	NS
35–30	NS	NS	NS	NS	NS	NS
Effect of Si on E
100–95	**	**	**	**	NS	**
80–75	**	**	**	**	NS	NS
55–50	**	**	**	**	**	NS
35–30	**	**	**	NS	NS	**
Effect of Si on gs
100–95	**	**	NS	**	**	**
80–75	NS	**	**	**	NS	NS
55–50	NS	NS	NS	NS	NS	**
35–30	NS	NS	**	NS	**	NS

DOI: 10.7717/peerj.10154/table-1

Notes:

The Saccharum officinarum plants were exposed to four soil water conditioNS (control, mild, moderate and severe drought, corresponding to available soil water capacity between 100–95%, 80–75%, 55–50% and 35–30% of the field capacity) and four levels of silicon (0: no Si, 100, 300 and 500 mg L⁻¹, n = 5 for each treatment).

** Significant variatioNS between Si applicatioNS at specific time of the day for particular soil water field capacity (ANOVA, P < 0.05).

NS, no significant difference.

Stomata are the main limiting factor for carbon dioxide and vapor water exchange between plant leaves and the atmospheric conditions thus gs was directly controlled by P_N and E. The diurnal changes of gs under all water limitations and Si treatments showed similar trends (Fig. 3). The relatively high gs levels were noted at 9:00–10:00, due to compensation for transpirational water loss before dark. Then, gs was significantly declined until evening (11:00–17:00). The gs was decreased with reducing soil moisture levels. The gs of plants receiving the 300 mg L⁻¹ was consistently up-regulated compared to that in the 500 mg L⁻¹ Si and that without Si application (Figs. 4A–4P). The impacts of Si application on gs under control and water stressed plants were statistically significant (P < 0.05) (Table 1).

Leaf E water loss was compensated at dusk per day. Hence, based on the diurnal changes of the main environmental variables affecting leaf E under limited and sufficient soil moisture levels (Figs. 5A–5P). Under well and limited irrigation, leaf E was increased at a relatively higher from 7:00 to 9:00, followed by a significant and continuous decline from 11:00 to 17:00, reaching the minimum level at 17:00 (Fig. 5). Irrespective of Si application, E decreased with decreasing soil moisture capacity at each observation point during the day. Amended Si significantly affected leaf E at 100–95%, 80–75%, 55–50% and 35–30% of available soil moisture (Table 1). The exogenous application of Si enhanced the leaf E for most of the day time, especially in the plants grown under limited water irrigation.

It may be seen from Figs. 3 to 5 that P_N, gs and E started increasing after the sun rise and reaches maximum between 9:00 and 10:00 and started declining thereafter. The model values of P_N, gs and E matches well with the observed values of diurnal variations of photosynthetic responses under water stress and Si application. The P_N under mild, moderate and severe stress conditions gradually increased with increasing levels of Si. The correlation coefficients (r) between P_N and water stress levels were found 0.987, 0.966, 0.978, 0.974 and 0.990–0.995, 0.956–0.966, 0.972–0.982, 0.968–0.973, respectively, in the treatments applied Si. Similarly diurnal variations of E were well explained by the model with value of r as 0.938–0.989 without Si and 0.928–0.981 with Si under limited water. Diurnal variation of g_s was also well explained by the model with r as 0.939–0.988 without Si and 0.911–0.988 under limited water with Si. Limited water supply drastically suppressed the photosynthetic responses of Saccharum officinarum plant leaves (Table S1).

Discussion

Sufficient water is important for the appropriate growth and development of plants. Its effect on plants depends on their developmental stage. Water stress is one of the major environmental factors that affect the plant development, cellular, metabolism, productivity and quality of Saccharum officinarum (Verma et al., 2019a, 2019b, 2020). Higher plants have developed different types of mechanisms to tolerate stresses. The protective roles of Si in combating various environmental stresses have been widely reported (Van Bockhaven, De Vleesschauwer & Höfte, 2013; Zhu & Gong, 2014; Verma et al., 2019a, 2020). In this study, the protective role of Si was investigated in Saccharum officinarum plants during insufficient water supply.

In our study, the growth and biomass of Saccharum officinarum plants were markedly down-regulated after the plants were subjected to stress. However, the application of Si with soil irrigation decreased the severity of water stressed growth inhibition. It enhanced Saccharum officinarum tolerance to water stress in terms of promoting the plant’s growth and development (Fig. 2). Previous research has shown that Si application could increase water stress tolerance of plants. However, most of the studies have been conducted on Si-accumulating plants, whereas less information is available regarding the role of Si on water deficit tolerance of plants (Nikolic et al., 2007). The increase in growth and biomass accumulation is endorsed to the higher P_N due to improved photosynthetic capacity of the stressed plants, which is in agreed with the previous demonstrations (Zhu & Gong, 2014; Liu et al., 2014; Shi et al., 2016; Verma et al., 2019a, 2020).

Our results further confirmed the findings of the previous reports that application of Si mitigated water stress and significantly affected plant growth and development in Oryza sativa (Ming et al., 2012), Zea mays (Kaya, Tuna & Higgs, 2006), Triticum aestivum (Pei et al., 2010; Gong & Chen, 2012), Sorghum bicolor (Liu et al., 2014), Solanum lycopersicum (Shi et al., 2016) and Saccharum officinarum (Verma et al., 2019a, 2020). Our results also imply a potential application of Si fertilizer in Saccharum officinarum crop production in tropical and sub-tropical regions.

In accordance with our experimental findings, leaf gas exchange significantly decreased during water stress with increasing stress levels (Figs. 3–5). Water deficit causes a major loss in LAE, LWC and photosynthetic pigments, which undeniably impairs and decreases P_N, directly affecting plant performance (Shi et al., 2016; Verma et al., 2019a; Liang et al., 2019). Si-mediated up-regulation of leaf P_N under drought could be attributed to improved or upgraded plant water status. In this experiment, the water status of Saccharum officinarum leaves were significantly improved by applied Si during drought. Our results showed that the applied Si mitigated the negative effects of water stress and enhanced the growth and biomass, improved the photosynthetic performance compared with the control plants during stress condition (Figs. 2–5). Our observations regarding enhancement in plant development and photosynthetic parameters due to exogenous use of Si during water stress condition are in accordance with other observations in various crops (Hattori et al., 2005; Pei et al., 2010; Ming et al., 2012; Shi et al., 2016; Chen et al., 2018; Ali et al., 2018; Verma et al., 2020). The improvement of growth by application of Si corresponded to the maintenance of higher P_N (Fig. 3).

Drought stress can severely decrease the yield of Saccharum officinarum by inhibiting the photosynthetic responses (Passioura, 2007; Santos et al., 2009; Verma et al., 2019b, 2019c). At the initial sign of drought stress, plants close the stomata to avoid excess water loss by E and as a consequence, under moderate stress condition, P_N is affected and is eventually inhibited by enhancing stress severity (Verma et al., 2019a, 2020). However, previous findings stated that an optimum level of Si fertilizer enhanced/improved the stomatal mechanisms/functions by enabling plants to reopen their stomata during water-deficit (Gong et al., 2005; Kaya, Tuna & Higgs, 2006; Chen et al., 2011; Liu et al., 2014; Verma et al., 2020). In this study, P_N was significantly low in the water-stressed plants without Si application, undoubtedly due to stomatal limitations. Si treatments, however, were found to escalate P_N and gs, and cause a simultaneous enhancement in LWC. The moderate to higher concentration of Si (300–500 mg L⁻¹) as soil irrigation were more effective in enhancing growth and photosynthetic performance during water stress condition (Figs. 2–5).

In this study, however, the E of Saccharum officinarum leaves was enhanced by applied Si under drought stress (Fig. 5). The supplied Si treatments also resulted in an enhancement of E, possibly driven by the increased gs to maintain a steady state of P_N against stress. Our results are in accordance with the previous studies like wheat, sorghum, rice, maize and tomato (Gong et al., 2005; Hattori et al., 2005; Chen et al., 2011; Shi et al., 2016) during abiotic stresses. Water deficiency and Si fertilization slightly affected the diurnal variations of Saccharum officinarum plants, which are positively correlated to the biological rhythm of the plants (Chen et al., 2016). However, compared with the plants cultivated under limited water without Si, Si generally enhanced plant growth and photosynthetic responses. An increase in soil moisture capacity is more effective than an increase in nutrient supply in improving the plant growth and development of Saccharum officinarum plants. Improvement of photosynthesis is the most important factor for overall plant performance. The change of PPFD during the natural diurnal cycle is the most important factor driving photosynthetic parameters (Paul & Pellny, 2003). Thus with maximum air RH and fully-irrigated plants, gas exchange changes in Saccharum officinarum plants is mainly driven by variations in sunlight intensity (Du et al., 2000), mainly resulting from the significantly direct or indirect dependance of C₄ photosynthetic enzymes to light intensity (Leegood & Walker, 1999; De Souza et al., 2018; Verma et al., 2019a). Stomata-related increment of P_N was found when Si improved gs and E (Chen et al., 2011; Savvas & Ntatsi, 2015; Verma et al., 2019b, 2019c).

The proposed model fitted quite well with the observed P_N, gs and E. The P_N fitted with “r” ranging from 0.956 to 0.995 and “S” 1.080 to 2.431; gs with “r” ranging from 0.911 to 0.988 and “S” from 6.540 to 22.276 and E with “r” from 0.928 to 0.989 and “S” from 0.062 to 0.324, respectively. The variations of model constants have a consistency in variations with changes in moisture status and applied Si levels (Figs. 3–5). The photosynthetic responses could be obtained for intermediate value of Si and moisture content of the soil by selecting the appropriate values of model constants. The model could be used to assess the photosynthetic advantages of Si application against water stress by integrating the model in terms of numerical values.

In summary, the results of this study revealed that application of Si might be an efficient approach for enhancing tolerance of Saccharum officinarum plants against water stress. It is also increased the growth, biomass accumulation and photosynthesis by protecting the leaf chlorophyl from degradation in Saccharum officinarum plants during stress. Further studies are needed to explore, how Si triggers the photosynthetic defense mechanism in Saccharum officinarum plants during drought stress. Thus, appropriate concentration is recommended for various crops to mitigate abiotic stresses.

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