Progress in the use of high-throughput screening technology for orthopedic diseases
Abstract
Orthopedic diseases are referred to as a series of conditions affecting the normal structure and function of the skeletal system. With a wide variety of types and a year-by-year increasing incidence, they have a severe impact on patients ’ quality of life. Traditional research has made relatively significant progress in understanding the pathogenesis and treating orthopedic diseases; however, it remains constrained by limitations such as low-throughput experimentation and difficulty in mimicking the complex bone microenvironment. In recent years, high-throughput screening (HTS) technology has been combined with experimental techniques and models ( such as microfluidics, omics technologies, 3D bioprinting, artificial intelligence, and organoid models ), which can be used to better simulate the real bone microenvironment and predict molecular activity. This combined approach has not only deepened our understanding of the mechanisms of orthopedic diseases but also broken through the limitations of traditional drug development, which further accelerates drug discovery and clinical translation. In this review, we focus on summarizing HTS technology and its contributions to the treatment of orthopedic diseases, aiming to enhance the understanding of HTS and promote its application in orthopedic disease therapy.