Scaling up DNA metabarcoding for freshwater macrozoobenthos monitoring

View preprint
2013 days ago
Huh. It looks like PeerJ also just links the DOI for each older version to the newest one. e.g. Version 3 https://t.co/ArmyHB9CL3 just redirects automatically to https://t.co/o2dSqjZSQQ which is version 5 and the most recent one.
reply repost on X like
2013 days ago
Huh. It looks like PeerJ also just links the DOI for each older version to the newest one. e.g. Version 3 https://t.co/ArmyHB9CL3 just redirects automatically to https://t.co/o2dSqjZSQQ which is version 5 and the most recent one.
reply repost on X like
RT @VascoElbrecht: The updated @PeerJPreprints of our #macroinvertebrate #metabarcodign paper is online (https://t.co/tIDCoCc29e)! Features…
reply repost on X like
RT @VascoElbrecht: The updated @PeerJPreprints of our #macroinvertebrate #metabarcodign paper is online (https://t.co/tIDCoCc29e)! Features…
reply repost on X like
2231 days ago
RT @VascoElbrecht: The updated @PeerJPreprints of our #macroinvertebrate #metabarcodign paper is online (https://t.co/tIDCoCc29e)! Features…
reply repost on X like
RT @metabar_papers: Scaling up DNA metabarcoding for freshwater macrozoobenthos monitoring https://t.co/QQmAWHZj9i
reply repost on X like
RT @VascoElbrecht: The updated @PeerJPreprints of our #macroinvertebrate #metabarcodign paper is online (https://t.co/tIDCoCc29e)! Features…
reply repost on X like
RT @metabar_papers: Scaling up DNA metabarcoding for freshwater macrozoobenthos monitoring https://t.co/QQmAWHZj9i
reply repost on X like
RT @metabar_papers: Scaling up DNA metabarcoding for freshwater macrozoobenthos monitoring https://t.co/QQmAWHZj9i
reply repost on X like
RT @metabar_papers: Scaling up DNA metabarcoding for freshwater macrozoobenthos monitoring https://t.co/QQmAWHZj9i
reply repost on X like
RT @VascoElbrecht: The updated @PeerJPreprints of our #macroinvertebrate #metabarcodign paper is online (https://t.co/tIDCoCc29e)! Features…
reply repost on X like
RT @metabar_papers: Scaling up DNA metabarcoding for freshwater macrozoobenthos monitoring https://t.co/QQmAWHZj9i
reply repost on X like
2234 days ago
RT @VascoElbrecht: The updated @PeerJPreprints of our #macroinvertebrate #metabarcodign paper is online (https://t.co/tIDCoCc29e)! Features…
reply repost on X like
2235 days ago
RT @VascoElbrecht: The updated @PeerJPreprints of our #macroinvertebrate #metabarcodign paper is online (https://t.co/tIDCoCc29e)! Features…
reply repost on X like
The updated @PeerJPreprints of our #macroinvertebrate #metabarcodign paper is online (https://t.co/tIDCoCc29e)! Features a slightly improved workflow, and great feedback from @BerndHaenfling. Thank you = ) Maybe soon accepted at Freshwater Biology
reply repost on X like
2235 days ago
Scaling up DNA metabarcoding for freshwater macrozoobenthos monitoring https://t.co/wIyl1C5yrl
reply repost on X like
Scaling up DNA metabarcoding for freshwater macrozoobenthos monitoring https://t.co/QQmAWHZj9i
reply repost on X like
Just submitted the updated preprint as well = ) should be available tomorrow. But for every one who can't wait, here is the slightly older version https://t.co/tIDCoCc29e
reply repost on X like
RT @VascoElbrecht: @eDNAScience @QIAGEN @dirch3 @BraukmannThomas @BiodiversityIn1 Thanks :D very happy with the 96 plate extractions and pc…
reply repost on X like
@eDNAScience @QIAGEN @dirch3 @BraukmannThomas @BiodiversityIn1 Thanks :D very happy with the 96 plate extractions and pcr. There is actually a sophisticated sample layout behind this, allowing to detect contamination and other things that might go wrong. See @PeerJPreprints: https://t.co/tIDCoCtDxO ☺️
reply repost on X like
@KristenFerns Maybe you find this video helpful concerning fusion primer design : ) https://t.co/k6q0EMdfW6 here is also the related @PeerJPreprints https://t.co/tIDCoCtDxO please let me know if i can help with anything #metabarcoding related : )
reply repost on X like
Getting things ready for 96 well plate extractions! =) Will use single tubes for digestions as the powder does too quickly disperse by static charging. But then switching to @Quiagen plate extractions after that. Scaling up #metabarcoding! @PeerJPreprints: https://t.co/tIDCoCc29e https://t.co/HhxiKqIBCV
reply repost on X like
@Gnarly_Larvae @dirch3 Yes, that’s probably the reason. The fusion primer system works brilliantly with 5% phiX, heterogeneity spacers (also used at in-line barcodes), and parallel sequencing. See also https://t.co/tIDCoCtDxO for recommendations : ) please let me know if you have any questions!
reply repost on X like
Hundreds of grinding chambers have arrived and @QIAGEN plate extraction kits for #metabarcoding at large scale! More information on scaling things up, here: https://t.co/tIDCoCc29e https://t.co/ZmCTAj0ZJh
reply repost on X like
A bit more shifting has to be done till for the 21 #metabarcoding primer sets to be tagged and tested = ) A Levenshtein distance of 1-2 bp means the primers are too similar -> Tagswitching can happen! scripts and more info in this related @PeerJPreprints: https://t.co/tIDCoCc29e https://t.co/KOm08QCFWL
reply repost on X like