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Scaling up DNA metabarcoding for freshwater macrozoobenthos monitoring

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RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
RT @VascoElbrecht: Updated version of our workflow manuscript now available @PeerJPreprints: Further improved tags and added analysis tools…
Updated version of our workflow manuscript now available @PeerJPreprints: Further improved tags and added analysis tools for Levenshtein distance to catch indels that might affect the tags! https://t.co/tIDCoCc29e
Happy to inform you that an updated version of the manuscript is live, solving the issues with the not ideal designed tags and adding Levenshtein distance analysis to account for indels in tags! https://t.co/Pvm6G6PMSD apologies again for overlooking this initially!
14 days ago
RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
43 days ago
RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
43 days ago
RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
44 days ago
RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
44 days ago
RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
44 days ago
RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
RT @VascoElbrecht: here is your weekend read: Scaling up #metabarcoding for monitoring macroinvertebrates! Practical guide to @illumina fus…
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Supplemental Information

Figure S1: Newly developed fusion primer sets (BF2+BR2), suitable for tagging 288 individual wells

DOI: 10.7287/peerj.preprints.3456v2/supp-1

Figure S2: Base composition of the inline tagging region

DOI: 10.7287/peerj.preprints.3456v2/supp-2

Figure S3: Hamming distance between tags for all fusion primers

DOI: 10.7287/peerj.preprints.3456v2/supp-3

Figure S4: Levenshtein distance between tags for all fusion primers

DOI: 10.7287/peerj.preprints.3456v2/supp-4

Script S1: R script used for randomly generating inline barcodes for the given primer sets (includes visualization, as shown in Figure S3 and Figure 3)

DOI: 10.7287/peerj.preprints.3456v2/supp-5

Table S1: Table providing an overview of proposed tagging combinations (as shown in Figure 3)

DOI: 10.7287/peerj.preprints.3456v2/supp-6

Manuscript file for providing feedback

(Pleas use track changes)

DOI: 10.7287/peerj.preprints.3456v2/supp-7

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Vasco Elbrecht conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Dirk Steinke reviewed drafts of the paper.

Funding

V.E. and D.S. are supported by funding through the Canada First Research Excellence Fund. This work represents a contribution to the ‘Food From Thought’ research program and the EU Action ‘DNAqua-Net’ (CA15219). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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