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The manuscript has improved over the review rounds and it is now accepted at PeerJ.
[# PeerJ Staff Note - this decision was reviewed and approved by Gerard Lazo, a PeerJ Section Editor covering this Section, who commented: 'The manuscript presents a good discussion and review of the literature for the plant antioxidant system. I agree that the manuscript is acceptable for publication. As it does not have any formal experiments performed, but does outline the key genes to look for in the pathways demonstrated, it would be valuable if a Supplemental table with KEGG and GO: terms be presented, to accompany the EC and MetaCyc terms presented in Table 1, as other research may use this manuscript as a citeable resource. I will leave this to the judgment of the academic editor, but do stress the value of this information as can be seen in the manuscript guidelines. This may be one of the better reviews that I’ve seen.'#]
Please take into consideration the reviewer's comments, and provide a revised manuscript and a detailed point-by-point rebuttal letter.
The manuscript is well written, professional English was used throughout the manuscript.
Basically the paper put together a revision of the plant antioxidant system in an integrative way. The main concepts are correctly explained according to up to date understanding of antioxidant enzyme families and antioxidant molecules. The major point is to look and integrate knowledge about this system at different cell locations, like the cytosol, mitochondria, chloroplast and peroxisomes.
In line 310, the name of the fungi is misspelled, is it Botrytis cinerea Pers.?
In line 323, the word arabidopsis should be Arabidopsis
In line 368, it says in chloroplasts associated, I suggest: .. in chloroplasts are associated ...
In line 441, do you mean ascorbate and glutathione are present or presented in vacuoles?
The method to select experimental work published through the years is clearly described after the Introduction.
Novel analysis of available information about the antioxidant system is presented. As part of this analysis the discussion is reasonable and authors synthesized the information in an organized way. Although the analysis was kind of historical, the length is appropiate to get a sense of how this field has advanced. It also, authors add insight to what could be done ahead to make more integrative studies.
I consider important the way they uncover current opportunities to set up enzymatic assays that are still lacking.
My suggestion is about information at Table 1, I am not sure about the relevance of the pathway link column, perhaps if this part is explained in the text it will help to understand how it can be helpful.
The paper is well written and the english language is good
Authors described their methodology and made a really good work of synthesis in the AOS components part. This paper also shed the light on modeling approaches to study antioxidant system and this is a very interesting part.
I only have a minor suggestion/modification: I was wondering why thioredoxins and glutaredoxins did not appear in the introduction part, in abbreviations and in table 1. First I was thinking that authors have forgotten these components since they talk about them in the main features of the antioxidant system components. Maybe authors could consider thioredoxins and glutaredoxins as the other components, because their choice of presnetation it is a little disturbing, as if thioredoxins and glutaredoxins were of less importance.
This is a very interesting review which summarizes much of the scientific knowledge about antioxidants systems in plants, putting together aspects of biochemistry, plants biology and subcellular integration.
The review is correctly organized in sections including different fields of the Antioxidant System (components, regulation, compartmentalization, modelling).). It is appreciable that a large bibliographic research was done, including both up-to-date and also some outdated bibliography
English grammar requires extensive editing. Authors should pay careful attention to some phrases or sentences which are confusing.
The survey methodology is very simple and concise and it is well described.
Bibliography is very abundant, covering all the aspects of the topics reviewed.
Some minor mistakes were found such as sources missing in the bibliography section and some citations incorrectly written.
Some suggestions were included in the attached pdf which, in my opinion, are important for the understanding of this review.
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