Differential expression of AtWAKL10 in response to nitric oxide suggests a putative role in biotic and abiotic stress responses

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Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials and Methods

Plant material, growth conditions and genotyping

Oxidative and nitrosative stress assay

Pathogen inoculations and cell death

Histological staining

Electrolyte leakage

Salt and drought stress

Quantitative real-time (qRT) PCR

Promoter analysis of AtWAKL10

Statistical analysis

Results

Loss of AtWAKL10 function results in differential responses to oxidative and nitrosative stress

AtWAKL10 is required for basal defense

AtWAKL10 positively regulates effector-triggered immunity

Promoter of AtWAKL10 rich in binding sites for stress response transcriptional regulators

AtWAKL10 positively regulates NaCl-induced salt stress

atwakl10 plants exhibit increased drought tolerance

Discussion

Conclusions

Supplemental Information

Original gel images presented in Fig. 1.

(A) Genotyping of atwalk10 kock out (KO) line. (B) Confirmation of KO line by studying gene expression in both wild type and KO line. (C) Actin levels in samples presented in (B).

DOI: 10.7717/peerj.7383/supp-1

Complete list of TFBs in the promoter region of AtWALK10.

DOI: 10.7717/peerj.7383/supp-2

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Phearom Bot performed the experiments.

Bong-Gyu Mun performed the experiments, analyzed the data, providing experimental tools.

Qari Muhammad Imran performed the experiments, analyzed the data, prepared figures and/or tables, authored drafts of the paper.

Adil Hussain conceived and designed the experiments, approved the final draft.

Sang-Uk Lee contributed in data analysis.

Gary Loake authored and reviewed drafts of the paper, approved the final draft.

Byung-Wook Yun conceived and designed the experiments, approved the final draft.

Data Availability

The following information was supplied regarding data availability:

Raw data are available in the Supplemental Files.

Funding

This research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education Grant Number NRF-2017R1A2B1008820. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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