All reviews of published articles are made public. This includes manuscript files, peer review comments, author rebuttals and revised materials. Note: This was optional for articles submitted before 13 February 2023.
Peer reviewers are encouraged (but not required) to provide their names to the authors when submitting their peer review. If they agree to provide their name, then their personal profile page will reflect a public acknowledgment that they performed a review (even if the article is rejected). If the article is accepted, then reviewers who provided their name will be associated with the article itself.
Thank you for supporting PeerJ and Open Publishing.
Both reviewers find your manuscript interesting and suitable for the PeerJ journal, however, they suggest several changes that may improve your manuscript. I would like to ask you to revise your manuscript accordingly and resubmit.
1. Correct miss-localization (page 4).
2. Correct coversplip (page 13)
3. Reference Format is not correct.
1. What do you mean with this sentence? The nuclear localization of the tagged proteins suggested association with chromosomes.
2. How many cells were analyzed for each variant of transfection with fluorescent/confocal microscope?
3. I miss scale bar for all microscopical images. What is it low and high magnification in Figure 4 & 5?
4. What kind of zoom factor was used in “low magnification”?
5. In Figure 2 D, S15-GFP image does not totally correspond to merge image.
6. Page 12: „Cells were incubated one ore two nights..“ Do you mean that cells were cultivated overnight and 30 hours?
1. Could you please suggest some future use of your results?
No Comments
No comments
No Comments
This is a careful study of the in vivo localisation of selected GFP-tagged ribosomal proteins in Drosophila salivary glands. The study supports the hypothesis that at least some ribosomal proteins are associated with chromatin in vivo. While not essential for publication, it would be good to know if the GFP-tagged RPs can rescue mutations in the endogenous genes. Similarly it would be interesting to examine the degree of co-localisation seen with different RPs, presumably why RFP and GFP versions were tagged.
All text and materials provided via this peer-review history page are made available under a Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.