MFAP2 enhances cisplatin resistance in gastric cancer cells by regulating autophagy

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Bioinformatics and Genomics

Main article text

 

Introduction

Materials and methods

Bioinformatics analysis

Cell culture

MTT assay

Western blot

Apoptosis assay

Cell transfection

Statistical analysis

Results

Database analysis

Differential expression analysis

Functional pathway enrichment analysis

Boxplot analysis of gene expression

Survival analysis

Clinical correlation analysis

ROC analysis

MFAP2 was upregulated in AGS/CDDP cells

Effect of MFAP2 on CDDP sensitivity of AGS

Effect of MFAP2 on the autophagy in AGS

MFAP2 enhanced CDDP resistance by promoting autophagy

Discussion

Conclusion

Supplemental Information

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Meng Li conceived and designed the experiments, performed the experiments, analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Hong-Yi Zhang conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Rong-Gui Zhang performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The gene expressions are available at GEO: GSE54129, GSE65801, GSE79973 and GSE103236.

The data from The Cancer Genome Atlas (TCGA) database is available at figshare: Zhang, Rong-Gui (2023): The specific data used from TCGA.zip. figshare. Dataset. https://doi.org/10.6084/m9.figshare.22820897.v2.

Funding

The authors received no funding for this work.

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