Serum expression of Vascular Endothelial-Cadherin, CD44, Human High mobility group B1, Kallikrein 6 proteins in different stages of laryngeal intraepithelial lesions and early glottis cancer

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Biochemistry, Biophysics and Molecular Biology

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Introduction

Materials and Methods

Patient cohort

Enzyme-linked immunosorbent assay (ELISA)

  • Human Vascular Endothelial-Cadherin Complex (VE-cad) (Sunred–Shanghai Sunred Biological Technology Co., Ltd; China; catalogue number: 201-12-0273; range: 0.15–40 ng/mL)

  • Human CD44 ELISA Kit (EIAab–Wuhan EIAab Science Co., Ltd; China; catalogue number: E0670h; range: 0.156–10 ng/mL)

  • Human High mobility group protein B1(HMGB1) ELISA Kit (Sunred–Shanghai Sunred Biological Technology Co., Ltd; China; catalogue number: 201-12-1636; range: 2–200 ng/mL)

  • Human Kallikrein 6 (KLK 6) ELISA Kit (Sunred–Shanghai Sunred Biological Technology Co., Ltd; China; catalogue number: 201-12-0863; range: 0.25–70 ng/mL)

Statistical analysis

Results

Discussion

Conclusions

Supplemental Information

Raw data of biomarker levels and characteristics of patients.

DOI: 10.7717/peerj.13104/supp-1

Exact values of test statistics, degrees of freedom and effect sizes.

* - Mann-Whitney U test

# - Kruskal Wallis one-way analysis of variance

DOI: 10.7717/peerj.13104/supp-2

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Michał Żurek conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Anna Rzepakowska conceived and designed the experiments, analyzed the data, prepared figures and/or tables, and authored or reviewed drafts of the paper approved the final draft.

Iwona Kotuła conceived and designed the experiments, performed the experiments, prepared figures and/or tables, and approved the final draft.

Urszula Demkow conceived and designed the experiments, and approved the final draft.

Kazimierz Niemczyk conceived and designed the experiments, and approved the final draft.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The study was conducted according to the guidelines of the Declaration of Helsinki and approved by the Bioethics Committee of Medical University of Warsaw (KB/71/2018). Informed written consents were obtained from all patients.

Data Availability

The following information was supplied regarding data availability:

The raw measurements are available in the Supplemental File.

Funding

The study was supported by the internal grant of Medical University of Warsaw (1WF/NM1/18). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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