The Broad Institute of MIT and Harvard
September 2017
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March 2022
Medical and Population Genetics
• First to perform single cell RNA (scRNA) sequencing and analysis in vascular tissue and identified unrecognized cellular heterogeneity
(Circulation, 2019)
• Developed and optimized high-throughput image-based assay to validate single cell RNA transcriptomic data (Circulation, 2019)
• Performed and analyzed single cell RNA sequencing and analysis in various human disease tissues and mouse models, including that of severe
COVID-19 (Nature, 2020)
• Performed downstream single cell RNA analysis and interpretation with integration of multimodality of data, including human population
genetics, such as GWAS data (Nature Genetics, 2021)
• Developed single cell nuclei sample preparation method for human vascular transcriptomic and epigenomic (ATAC-seq) evaluation.
• Performed spatial transcriptome: Slide-seq & visium
• Developed next-generation sequencing based assays and analysis pipeline to study somatic variation in diseased human vascular tissue.
• Performed Genome Wide association studies (GWAS), rare variant association studies and burden analysis (RVAS), polygenic risk score
estimation of both quantitative and qualitative traits.
• Developed analysis pipeline for high-content imaging (cell painting) and single cell RNA-sequencing CRISPR screen in endothelial cells
(Perturb-seq)
• Developed high efficiency method for genome editing in primary human vascular cells (Current Protocols, 2021
