Microbiology

Academic Editor Commentary

Effects of preservation method on canine (Canis lupus familiaris) fecal microbiota (2018) PeerJ

Konstantinos Kormas –– Sampling and handling of samples is very crucial in -omics based studies and very often the necessary attention is not met in publiched works. Such studies are necessary... –– read more

Sequencing 16S rRNA gene fragments using the PacBio SMRT DNA sequencing system

Editor rating: 7 / 10

Christine Josenhans –– platform comparison for NGS high throughput sequencing; implementation of novel platform based on PacBio SMRT sequencing for 16S amplicon sequencing. These technologies are of prime importance in the ever-growing field of microbiome/microbiota research
H2-saturation of high affinity H2-oxidizing bacteria alters the ecological niche of soil microorganisms unevenly among taxonomic groups

Editor rating: 7 / 10

Maria Rosaria Corbo –– The results of this paper demonstrating that H2-rich environments exert a direct influence on soil H2-oxidizing bacteria in addition to indirect effects on other members of the bacterial communities can be considered a significant contribution about the knowledge in the field.
Comparison of three clustering approaches for detecting novel environmental microbial diversity

Editor rating: 7 / 10

Gilles van Wezel –– The paper compares approaches to analyse microbial biodiversity across different samples. The work thereby provides insights to scientists into which approach may best suit their needs.
Unravelling potential virulence factor candidates in Xanthomonas citri. subsp. citri by secretome analysis

Editor rating: 7 / 10

Gerard Lazo –– Though focused on limited samples, the initial premise to build methodology to study the secretome may help expand approaches to screen additional mutants and help catalog genes important in the plant-microbe interaction. This work also addresses agriculturally important research.
Characterisation of the human uterine microbiome in non-pregnant women through deep sequencing of the V1-2 region of the 16S rRNA gene

Editor rating: 8 / 10

Offer Erez –– The understanding of the human microbiom in immune privilige sites is important and tyhis manuscript contribute substantially to this field
One-step multiplex real-time RT-PCR assay for detecting and genotyping wild-type group A rotavirus strains and vaccine strains (Rotarix® and RotaTeq®) in stool samples

Editor rating: 7 / 10

Ravi Tandon –– The article is important because it describes about detecting different types of Rotavirus in the stool samples. This assay will allow others to detect the virus using the one-step multiplex real-time PCR assay without doing cDNA synthesis and the PCR, and will help others in their ongoing research.
Occurrence of ferredoxin:NAD+ oxidoreductase activity and its ion specificity in several Gram-positive and Gram-negative bacteria

Editor rating: 7 / 10

Gilles van Wezel –– The work provides evience on the genetic basis for ferredoxin:NAD+ oxidoreductase bioactivity in different bacterial systems.
The role of 2,4-dihydroxyquinoline (DHQ) in Pseudomonas aeruginosa pathogenicity

Editor rating: 7 / 10

Timothy Read –– Novel role for quorum sensing in Pseudomonas pathogenicity.
Swarm v2: highly-scalable and high-resolution amplicon clustering

Editor rating: 7 / 10

Gilles van Wezel –– the update of Swarm will hopefully be a useful bioinformtic tool for those in the field of amplicon sequencing and clustering.
Persistent microbial dysbiosis in preterm premature rupture of membranes from onset until delivery

Editor rating: 7 / 10

Offer Erez –– The article further elucidate the host environment interaction in preterm parturition this is important topic that better understanding in the future may enable us to develop new treatment modalities for the prevention of these complications.