Biochemistry, Biophysics and Molecular Biology

Evidence of transfer of miRNAs from the diet to the blood still inconclusive
Alberto Davalos –– The manuscript revises the many obstacles and challenges, from a historic perspective, for the possible notion of miRNA transfer from one specie to another.
Comparison of serum exosome isolation methods on co-precipitated free microRNAs
Gwyn Gould –– ECVs are an increasingly important area of research; this adds to the methodological literature comparing different methods of isolating these vesicles from serum and analysing the issue of associated RNA species.
Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli
Walter de Azevedo Jr. –– In this work conducted by Wang et al. the authors described a novel biological control approach for manipulating the growth of a pathogenic microorganism. The authors cloned a gene encoding "Aspergillus niger" ZM-8 glucose oxidase and transferred it to "Pichia pastoris" to form a transgenic strain GS115-His-GOD. Functional comparison of transgenic "P. pastoris" GS115-His-GOD with non-transgenic GS115-pPIC9K clearly showed that the transgenic sample could express glucose oxidase by methanol induction. Also, this study indicated that the hydrogen peroxide could be generated with glucose as the substrate during the fermentation process, and showed significant inhibition of the growth of Agrobacterium and "Escherichia coli" in logarithmic phase.
Proteins from Lignosus tigris with selective apoptotic cytotoxicity towards MCF7 cell line and suppresses MCF7-xenograft tumor growth
Motoki Takaku –– The authors identified anticancer properties of the Lignosus tigris sclerotia.
DNA from mollusc shell: a valuable and underutilised substrate for genetic analyses
Xavier Pochon –– This study showed that find that reasonable quantities of DNA can be derived from aged, beach-cast and cooked mussel (Perna canaliculus) shell for routine PCR analyses of mitochondrial and nuclear gene fragments.
Identification of novel mutations in RNA-dependent RNA polymerases of SARS-CoV-2 and their implications on its protein structure
Joseph Gillespie –– Contributes to the landscape of naturally occurring variability in SARS-CoV-2 genomes
Conformational heterogeneity of Savinase from NMR, HDX-MS and X-ray diffraction analysis
Emanuele Paci –– Important comparison of structural and dynamical information using orthogonal techniques
qPCR multiplex detection of microRNA and messenger RNA in a single reaction
Rogerio Sotelo-Mundo –– This novel method of mRNA and miRNA has lots of potential for application in many areas of biological research.
Marsupial and monotreme milk—a review of its nutrient and immune properties
Grant McClelland –– This comprehensive review brings together current knowledge of marsupial and monotreme milk. This will provide a valuable resource for those interested in maternal care in mammals.
Biochemical parameters, dynamic tensiometry and circulating nucleic acids for cattle blood analysis: a review
Zekiye Altun –– This review is related to veterinary biochemistry and circulating nucleic acids. This review will help at this research area to researchers.
Biochemistry, Biophysics and Molecular Biology

Section discussions

The combination of lactoferrin and linolenic acid inhibits colorectal tumor growth through activating AMPK/JNK-related apoptosis pathway @thePeerJ

Paeoniflorin improves functional recovery through repressing neuroinflammation and facilitating neurogenesis in rat stroke model @thePeerJ

First body of evidence suggesting a role of a tankyrase-binding motif (TBM) of vinculin (VCL) in epithelial cells @thePeerJ

New article @PeerJLife #Biodiversity #Biogeography #MarineBiology #MolecularBiology