Biochemistry, Biophysics and Molecular Biology

A simple and efficient cloning system for CRISPR/Cas9-mediated genome editing in rice
Kun Lu –– CRISPR-Cas9 based genome editing has become the method of choice for the rapid generation of transgenic plants. We hope that the fast and efficient cloning system the authors presented could facilitate genetic engineering in plants.
Flavonoids from Epimedium pubescens: extraction and mechanism, antioxidant capacity and effects on CAT and GSH-Px of Drosophila melanogaster
Chin Ping Tan –– This paper highlights work related to ultrasound extraction of flavonoids from Epimedium pubescens and subsequent evaluation on antioxidant properties and effect of the extract on fruit flies.
DNA methylation profiling in recurrent miscarriage
Vladimir Uversky –– This study can be useful for better understanding of the molecular basis of recurrent miscarriage
Icaritin, an inhibitor of beta-site amyloid cleaving enzyme-1, inhibits secretion of amyloid precursor protein in APP-PS1-HEK293 cells by impeding the amyloidogenic pathway
Paul Tulkens –– This paper shows that an aprenylflavonoid derivative from Epimedium brevicornum Maxim may have neuroprotective effects. Icaritin, an inhibitor of beta-site amyloid cleaving enzyme-1, inhibits indeed the secretion of amyloid precursor protein in APP-PS1-HEK293 cells by impeding the amyloidogenic pathway. This effect, however, is only studied in vitro, calling for further studies demonstrating its potential in vivo.
Design and preliminary application of affinity peptide based on the structure of the porcine circovirus type II Capsid (PCV2 Cap)
Omar Gonzalez-Ortega –– Improvements in protein purification are always relevant. The in silico procedure followed is interesting.
Propofol inhibits stromatoxin-1-sensitive voltage-dependent K+ channels in pancreatic β-cells and enhances insulin secretion
Vladimir Uversky –– The manuscript reports important data and definitely will have a noticeable impact.
Transcriptome profiling analysis reveals key genes of different coat color in sheep skin
Cong-Jun Li –– The wool of sheep is a crucial product financially for farmers. Understanding the genetic regulation of wool color is very important as it can be the necessary information for the genetic selection of sheep breed
Magnetically directed antioxidant and antimicrobial agent: synthesis and surface functionalization of magnetite with quercetin
Eder Lenardao –– The combined properties of the new nanocomposite with quercetin makes it a promising candidate for application in biomedicine. It's the first report on the combination of the natural bioactive flavonoid quercetin with magnetite nanoparticles.
New sources of Sym2A allele in the pea (Pisum sativum L.) carry the unique variant of candidate LysM-RLK gene LykX
Yuriy Orlov –– The work presents additional pea lines with a Sym2A allele. It highlights ligand receptor compatibility determining rhizobial nodulation of legumes.
Characterization of aminopeptidase encoding gene anp-1 and its association with development in Caenorhabditis elegans
Juan Riesgo-Escovar –– I think this article will be important for people working on C. elegans development, at least.
Biochemistry, Biophysics and Molecular Biology

Section discussions

100 visitors to my 'Fast computational mutation-response scanning of proteins' article published in #OpenAccess journal @thePeerJ

16 days ago
Semidry acid hydrolysis of cellulose sustained by autoclaving for production of reducing sugars for bacterial biohydrogen generation from various cellulose feedstock Researchers @taibahu carried out hydrolysis in loosely screw capped 250 ml DURAN® bottles

Long non-coding RNA taurine upregulated gene 1 is downregulated in osteoporosis and influences the osteogenic differentiation of bone marrow mesenchymal stem cells @thePeerJ

Research from @CUBoulder examines the widely assumed phenotypic associations in #Cannabis #sativa discovering a lack of shared genetic basis Read the full @PeerJLife article #Cannabinoid #Chemotype #Hemp #Marijuana

Historical effective population size of North American hoary bat (Lasiurus cinereus) and challenges to estimating trends in contemporary effective breeding population size from archived samples @thePeerJ

23 days ago
Our article describing a new species of #Elatostema from limestone #karst in #Guangxi, China and its chloroplast genome, published today @thePeerJ @KewScience @KewAmericas #Urticaceae #Taxonomy