Biochemistry, Biophysics and Molecular Biology

Comparing sediment DNA extraction methods for assessing organic enrichment associated with marine aquaculture
Owen Wangensteen –– A well-designed study which compares biodiversity results obtained from different DNA extraction methods for marine sediments. This comparison is crucial to help marine ecologists to make the right method choice in future molecular biodiversity studies.
Differences in small noncoding RNAs profile between bull X and Y sperm
Korakot Nganvongpanit –– This work has shown the different sncRNA profiles of X and Y sperm in cattle and understanding of their potential roles in the regulation of sex differences in sperm and early embryonic development.
Evidence of transfer of miRNAs from the diet to the blood still inconclusive
Alberto Davalos –– The manuscript revises the many obstacles and challenges, from a historic perspective, for the possible notion of miRNA transfer from one specie to another.
Comparison of serum exosome isolation methods on co-precipitated free microRNAs
Gwyn Gould –– ECVs are an increasingly important area of research; this adds to the methodological literature comparing different methods of isolating these vesicles from serum and analysing the issue of associated RNA species.
Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli
Walter de Azevedo Jr. –– In this work conducted by Wang et al. the authors described a novel biological control approach for manipulating the growth of a pathogenic microorganism. The authors cloned a gene encoding "Aspergillus niger" ZM-8 glucose oxidase and transferred it to "Pichia pastoris" to form a transgenic strain GS115-His-GOD. Functional comparison of transgenic "P. pastoris" GS115-His-GOD with non-transgenic GS115-pPIC9K clearly showed that the transgenic sample could express glucose oxidase by methanol induction. Also, this study indicated that the hydrogen peroxide could be generated with glucose as the substrate during the fermentation process, and showed significant inhibition of the growth of Agrobacterium and "Escherichia coli" in logarithmic phase.
Proteins from Lignosus tigris with selective apoptotic cytotoxicity towards MCF7 cell line and suppresses MCF7-xenograft tumor growth
Motoki Takaku –– The authors identified anticancer properties of the Lignosus tigris sclerotia.
DNA from mollusc shell: a valuable and underutilised substrate for genetic analyses
Xavier Pochon –– This study showed that find that reasonable quantities of DNA can be derived from aged, beach-cast and cooked mussel (Perna canaliculus) shell for routine PCR analyses of mitochondrial and nuclear gene fragments.
Identification of novel mutations in RNA-dependent RNA polymerases of SARS-CoV-2 and their implications on its protein structure
Joseph Gillespie –– Contributes to the landscape of naturally occurring variability in SARS-CoV-2 genomes
Conformational heterogeneity of Savinase from NMR, HDX-MS and X-ray diffraction analysis
Emanuele Paci –– Important comparison of structural and dynamical information using orthogonal techniques
qPCR multiplex detection of microRNA and messenger RNA in a single reaction
Rogerio Sotelo-Mundo –– This novel method of mRNA and miRNA has lots of potential for application in many areas of biological research.
Biochemistry, Biophysics and Molecular Biology

Section discussions

Wang et al. examine how barley grain size impacts storage capacity during grain filling with the use of molecular markers Read the full @PeerJLife article #AgriculturalScience #Genetics #MolecularBiology #PlantScience

Kim et al. @SNUnow reveal a new coral barnacle exclusively associated with the high latitude coral Alveopora japonica in the waters of southern Korea after DNA-based diversity assessment Read the full @PeerJLife article #Biodiversity #MarineBiology

Gilda Valiero's article has been published today in @thePeerJ #Bioinformatics #Microbiology #MolecularBiology Congratulations !!!

Phenotypic, molecular and pathogenic characterization of Colletotrichum scovillei infecting Capsicum species in Rio de Janeiro, Brazil @thePeerJ

Comparative transcriptome and histological analyses provide insights into the skin pigmentation in Minxian black fur sheep (Ovis aries) @thePeerJ

Transcriptomics analysis reveals the effect of Broussonetia papyrifera L. fermented feed on meat quality traits in fattening lamb @thePeerJ

Artículo original de la LCF: Dysregulation of miR-381-3p and miR-23b-3p in skeletal muscle could be a possible estimator of early post-mortem interval in rats. Disponible en: