Biochemistry, Biophysics and Molecular Biology

15 April 2021

Development of a single-chain fragment variable fused-mutant HALT-1 recombinant immunotoxin against G12V mutated KRAS colorectal cancer cells

Michelle Yee Mun Teo, Jeremy Jeack Ceen Ng, Jung Yin Fong, Jung Shan Hwang, Adelene Ai-Lian Song, Renee Lay Hong Lim, Lionel Lian Aun In

Maria Cristina Albertini –– Important results have been found with advances in colorectal cancerogenesis.

7 April 2021

Effect of leaf position and days post-infiltration on transient expression of colorectal cancer vaccine candidate proteins GA733-Fc and GA733-FcK in Nicotiana benthamiana plant

Kibum Kim, Yang Joo Kang, Se Ra Park, Do-Sun Kim, Seung-Won Lee, Kinarm Ko, Daniel Ponndorf, Kisung Ko

Abhiram Maddi –– Production of vaccines in plants may be an important approach in the future as we deal with the current pandemic.

23 March 2021

The effect of carbon monoxide on meiotic maturation of porcine oocytes

David Němeček, Eva Chmelikova, Jaroslav Petr, Tomas Kott, Markéta Sedmíková

Judith Yanowitz –– The impact of carbon monoxide on oocyte maturation has the potential to change in vitro culturing methodology to increase oocyte quality.

15 March 2021

Preparation and characterization of gelatin-polysaccharide composite hydrogels for tissue engineering

Jing Ye, Gang Yang, Jing Zhang, Zhenghua Xiao, Ling He, Han Zhang, Qi Liu

Omar Gonzalez-Ortega –– The potential final application is attractive. Despite this, there is still work to be done to "optimize" the composite gel.

10 February 2021

Exploring the occurrence of thioflavin-T-positive insulin amyloid aggregation intermediates

Mantas Ziaunys, Andrius Sakalauskas, Kamile Mikalauskaite, Vytautas Smirnovas

Eugene Permyakov –– Any new information on insulin properties is of importance for medicine.

15 January 2021

Effective ribosomal RNA depletion for single-cell total RNA-seq by scDASH

Danson S.C. Loi, Lei Yu, Angela R. Wu

Joseph Gillespie –– Loi et al. describe a novel approach for performing rRNA depletion in single cell sequencing experiments that facilitates detection of non-polyadenylated and partially degraded RNAs.

5 January 2021

Research on the measurement of intracranial hemorrhage in rabbits by a parallel-plate capacitor

Zelin Bai, Haocheng Li, Jingbo Chen, Wei Zhuang, Gen Li, Mingsheng Chen, Jia Xu, Shuanglin Zhao, Yuening Liu, Jian Sun, Feng Wang, Lin Xu, Mingxin Qin, Gui Jin

Jafri Abdullah –– More portable star trek like scanning equipment are important for screening blood clots in the brains are needed

4 January 2021

Identification and molecular characterization of mutations in nucleocapsid phosphoprotein of SARS-CoV-2

Gajendra Kumar Azad

Timothy Newsome –– Understanding the significance of SARS-CoV-2 genomic changes is key to an effective response in the current pandemic. This new research identified the N-protein as a mutational hotspot, with potential implications to a number of aspects of virus replication.

21 December 2020

Cinnamtannin B1 attenuates rosacea-like signs via inhibition of pro-inflammatory cytokine production and down-regulation of the MAPK pathway

Hung-Lin Kan, Chia-Chi Wang, Yin-Hua Cheng, Chi-Lung Yang, Hsun-Shuo Chang, Ih-Sheng Chen, Ying-Chi Lin

Consolato Sergi –– Cinnamtannin B1 is a tannin found in Cinnamomum verum and has been found to have pharmacological effects in treating type 2 diabetes mellitus and insulin resistance. This article brilliantly targets the attenuation of rosacea-like symptoms by investigating the MAPK pathway.

21 December 2020

Improved plaque assay for human coronaviruses 229E and OC43

Nicole Bracci, Han-Chi Pan, Caitlin Lehman, Kylene Kehn-Hall, Shih-Chao Lin

Rogerio Sotelo-Mundo –– The method proposed by Bracci et al. provides a practical assay to evaluate antiviral compounds using a cellular assay that does not rely on reverse transcriptase-PCR. The use of milder human coronavirus strains HCoV-229E and OC43 in BSL-2 biosafety conditions will permit the evaluation of potential drugs in an in vitro antiviral assay.