Preprints (not yet peer-reviewed)

241 downloads
561 views

Microbes for environmental research should be cultured in growth media with characteristics (e.g., pH, ionic strength, and ionic composition) as close to their original habitat as possible. Additionally, the medium should also enable high cell density to be obtained,...

["Biochemistry","Bioengineering","Biotechnology","Environmental Sciences","Microbiology"]
doi:10.7287/peerj.preprints.1406v5
932 downloads
644 views

Microbes, with their vast metabolic capabilities and great adaptability, occupy almost every conceivable ecological niche on Earth. Thus, could they survive in the oligotrophic (i.e., nutrient poor) deionized (DI) water that we use for experiments? Observations...

["Biochemistry","Biodiversity","Ecology","Environmental Sciences","Microbiology"]
doi:10.7287/peerj.preprints.181v5
1,004 downloads
1,812 views

Zeta potential, defined as the electric charge at the shear plane, is widely used as a proxy parameter for bacterial cell surface charge. Nonspecific adsorption of ions or polyelectrolytes onto the cell surface, however, alters the value and polarity of the measured...

["Bioengineering","Biophysics","Biotechnology","Environmental Sciences","Microbiology"]
doi:10.7287/peerj.preprints.110v6
189 downloads
867 views

Solid medium enables spatially resolved simultaneous cultivation of different microbes in a simple and relatively low cost format useful for preliminary screening of microbial diversity. Lack of color contrast between colonies and agar, however, hampers colony...

["Biochemistry","Bioengineering","Environmental Sciences","Microbiology"]
doi:10.7287/peerj.preprints.89v5
109 downloads
528 views

Bacteria surface charge mediates important cell-environment and microbe-host interactions, and its accurate and precise measurement by microelectrophoresis requires removing metabolites adhered to the cell surface, where repeated centrifugation and washing by buffers...

["Biochemistry","Biotechnology","Environmental Sciences","Microbiology"]
doi:10.7287/peerj.preprints.91v5
52 downloads
254 views

This paper presents the ecotoxicological assessment and environmental risk evaluation of complex pesticide mixtures occurring in freshwater ecosystems in southern Sweden. The evaluation is based on exposure data collected between 2002 and 2013 by the Swedish pesticide...

["Agricultural Science","Environmental Sciences"]
doi:10.7287/peerj.preprints.2779v2
12 downloads
79 views

Background. The effluents of the mono-specific aquaculture contain high concentrations of dissolved nutrients and organic matter, which affect negatively water quality of the recipient aquatic ecosystems. A key feature of water quality is its transparency. Chromophoric...

["Aquaculture, Fisheries and Fish Science","Environmental Sciences"]
doi:10.7287/peerj.preprints.3012v1
181 downloads
822 views

The characteristics of the culture vessel determine, to a large extent, the type of growth medium suitable for use. For example, most growth media for high cell density cultivation are designed for expensive bioreactors operating either in continuous or fed-batch...

["Biochemistry","Bioengineering","Biotechnology","Environmental Sciences","Microbiology"]
doi:10.7287/peerj.preprints.117v5
302 downloads
1,266 views

Sufficient quantities of cells of consistent characteristics are needed for studying biologicalprocesses (at the population level ) in many areas of applied microbiology. However, generating the requisite biomass by cell culture is usually the rate-limiting step...

["Biochemistry","Biotechnology","Environmental Sciences","Microbiology"]
doi:10.7287/peerj.preprints.115v6
246 downloads
1,070 views

Environmental bulk samples often contain many taxa that vary several orders of magnitude in biomass. This can be problematic in DNA metabarcoding and metagenomic high-throughput sequencing approaches, as large specimens contribute disproportionately high amounts...

["Biodiversity","Conservation Biology","Environmental Sciences","Molecular Biology","Zoology"]
doi:10.7287/peerj.preprints.2561v3
15 downloads
100 views

DNA extracted from feces (human and bovine) and water samples was used for the massive pyrosequencing of the hypervariable V3 region of the 16S rRNA gene, revealing 4296 operational taxonomic units (OTUs). The greatest diversity was observed in samples of cattle...

["Biodiversity","Environmental Sciences","Genomics","Microbiology"]
doi:10.7287/peerj.preprints.2992v1
64 downloads
359 views

Primary production due to photosynthesis results in daytime oxygen production across marine and freshwater ecosystems. However, a prevalent, globally-occurring nighttime spike in dissolved oxygen (DO) challenges our traditional assumption that oxygen production...

["Biochemistry","Ecology","Ecosystem Science","Environmental Sciences","Marine Biology"]
doi:10.7287/peerj.preprints.2935v2
38 downloads
162 views

The use of multiple sampling areas in landscape genetic analysis has been recognized as a useful way to generalize the patterns of environmental effects on gene flow. It allows reducing the variability of inference, accounting for multiple scales and locations...

["Entomology","Environmental Sciences","Molecular Biology"]
doi:10.7287/peerj.preprints.2968v1
31 downloads
159 views

Texas is the second largest state in the United States of America, and the largest state in the contiguous USA at nearly 700,000 sq. km. Several Texas bumble bee species have shown evidence of declines in portions of their continental ranges, and conservation initiatives...

["Ecology","Ecosystem Science","Entomology","Environmental Sciences"]
doi:10.7287/peerj.preprints.2932v1
22 downloads
389 views

Algae with potential biotechnological applications in different industries are commonly isolate from the environment in order to obtain pure (axenic) stocks that can be safely stored for long periods of time. To obtain axenic cultures, antibiotics are frequently...

["Aquaculture, Fisheries and Fish Science","Environmental Sciences"]
doi:10.7287/peerj.preprints.2881v2
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