Preprints (not yet peer-reviewed)

Tea is one of three major non-alcoholic beverages that are popular all around the world. The economic value of the final tea product largely depends on the post-harvest physiology of tea leaves. The utilization of quantitative reverse transcription polymerase chain...

["Agricultural Science","Biotechnology","Molecular Biology"]
doi:10.7287/peerj.preprints.27287v1
48 downloads
171 views

The gut microbiota has been shown to have an important influence on host health. Microbial composition of the gut microbiota is modulated by diet and human habits. The composition of human gut microbiota changes with age; and alterations in this composition may...

["Biotechnology","Genomics","Microbiology","Molecular Biology"]
doi:10.7287/peerj.preprints.27252v1
72 downloads
133 views

Growing microorganisms for laboratory experiments or industrial biotechnological process is an activity which involves the use of bioreactors. Although there are many commercially available equipment, most of them lack the flexibility of an open-source solution....

["Bioinformatics","Biotechnology"]
doi:10.7287/peerj.preprints.27150v1
55 downloads
100 views

Microbes live in communities known as biofilm on many surfaces. Thus, understanding the spatially-resolved intercellular communication and signalling link would be important to elucidate the fundamental mechanisms that govern the division of labour within biofilm...

["Biochemistry","Bioengineering","Biotechnology","Cell Biology","Microbiology"]
doi:10.7287/peerj.preprints.27117v1
118 downloads
286 views

The New Zealand Environmental Protection Authority (EPA) issued a Decision that makes the use of externally applied double-stranded (ds)RNA molecules on eukaryotic cells or organisms technically out of scope of legislation on new organisms, because in its view...

["Biodiversity","Biotechnology","Molecular Biology","Legal Issues"]
doi:10.7287/peerj.preprints.27108v1
46 downloads
113 views

The WD40 gene family in Prunusmume was identified by bioinformatics analysis., The evolution and function of WD40 gene family were predicted by phylogenetic analysis, chromosome localization analysis, protein sequence analysis, exon-intron structure analysis. The...

["Biotechnology","Genetics","Genomics"]
doi:10.7287/peerj.preprints.27106v1
1,007 downloads
3,383 views

In an organism, be it plant, animal or human, almost every gene has its own promoter sequence, which is typified as a DNA stretch that controls how a gene is expressed in a cell. Hence, the activity of a promoter controls in which cell type, during which developmental...

["Agricultural Science","Biotechnology","Genetics","Plant Science","Virology"]
doi:10.7287/peerj.preprints.27096v2
81 downloads
152 views

Starch branching enzyme (SBE), which is one of the key enzymes associated with amylopectin biosynthesis, plays important roles in variable biological processes. Despite its importance, SBE is rarely studied in the banana (Musa acuminata L.) which is a typical starchy...

["Agricultural Science","Biotechnology","Molecular Biology","Plant Science"]
doi:10.7287/peerj.preprints.27045v1
43 downloads
109 views

Large culture volume in bioreactor necessitates aeration for providing sufficient oxygen for cell growth. Thus, extend of aeration and amount of anti-foam needed for suppressing foam formation are key parameters determining the success of bioreactor fermentation....

["Biochemistry","Bioengineering","Biotechnology","Cell Biology","Microbiology"]
doi:10.7287/peerj.preprints.27014v1
739 downloads
1,405 views

Many pressing medical challenges - such as diagnosing disease, enhancing directed stem cell differentiation, and classifying cancers - have long been hindered by limitations in our ability to quantify proteins in single cells. Mass-spectrometry (MS) is poised to...

["Bioengineering","Biotechnology","Cell Biology","Computational Biology","Genomics"]
doi:10.7287/peerj.preprints.26821v2
35 downloads
132 views

Intact biomolecules carry its identity through its atomic constituents and mass, while fragmented biomolecules require reconstruction for their identity to be retrieved. Hence, for profiling biomolecules in mixtures, the goal would be the gentle ionization of biomolecules...

["Biochemistry","Bioengineering","Biophysics","Biotechnology","Cell Biology"]
doi:10.7287/peerj.preprints.26983v1
97 downloads
255 views

Clustered regularly interspersed short palindromic repeats (CRISPR) is a natural defense system for bacteria and archaea against foreign DNA and RNA. Specifically, short snippets of foreign DNA or RNA are incorporated into protospacer adjacent motif (PAM) repeats...

["Biochemistry","Bioengineering","Biotechnology","Microbiology","Molecular Biology"]
doi:10.7287/peerj.preprints.26975v1
51 downloads
162 views

Growth in minimal salts medium meant that a microorganism has the full repertoire of biosynthetic machinery for surviving in habitats lacking in vitamins and growth factors. This significantly expands the types of habitats suitable for colonization by the microbe....

["Biochemistry","Biotechnology","Cell Biology","Ecology","Microbiology"]
doi:10.7287/peerj.preprints.26970v1
183 downloads
188 views

Overweight and obesity are considered as a major cause of various conditions related to metabolic syndrome. Yet, considering the complex interacting factors leading to pathogenicity and underlying mechanisms, it remains a poorly defined area. Some probiotics have...

["Biotechnology","Microbiology"]
doi:10.7287/peerj.preprints.26959v1
234 downloads
141 views

LB Lennox medium is commonly used for bacterial cell culture, but the amount of biomass obtained while sufficient for molecular biology and biochemical studies, is insufficient for applied microbiology research. Thus, supplementation of LB Lennox with glucose is...

["Biochemistry","Bioengineering","Biotechnology","Cell Biology","Microbiology"]
doi:10.7287/peerj.preprints.26949v1
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