[Experimental] List of manuscripts available for review volunteers
2 manuscripts available for review volunteers
June 10, 2017
In order to understand the migratory phenotype of a long-distance migrant, the Northern Wheatear (Oenanthe oenanthe), in terms of genetics, gene expression, biochemistry and physiology, we have established a first annotated transcriptome from sequence data (RNASeq) generated via the Roche GS FLX System. The transcriptome was constructed de novo from pooled mRNA of six organs of nine birds: brain, muscle, intestine, liver, adipose tissue and skin. All individuals were captive birds, and the samples were collected at three different time points before the onset of migration. We were able to identify 14,724 genes (17,539 isogroups and 21,982 isotigs), 63% of them with more than 50% identity with other genes from genomes of other birds, such as Turkey and Zebra Finch, or to proteins in the Swissprot and NCBI databases. Around 13% of the isogroups have a putative splicing variant, most of them just a second alternative.
May 27, 2017
Plant type III polyketide synthase can catalyze into a series of secondary metabolites with different structures and different biological functions, and play a very important role in plant growth, development and resistance to stress. At present, the PKS gene has been identified and studied in a variety of plants. Here, we identified 11 PKS genes from upland cotton and compared them with 41 PKS genes in Populus tremula, Vitis vinifera, Malus domestica and Arabidopsis thaliana. According to phylogenetic tree, a total of 52 PKS genes can be divided into four subfamilies (I-IV). The analysis of gene structures and conserved motifs revealed that most of the PKS genes were composed of two exons and one intron, and have two characteristic conserved domains of the PKS gene family. The results of chromosome localizations showed that the distribution of PKS genes in these five species was irregular. Through the analysis of Ka/Ks ratio, we determined that the selection of purification is of great significance in maintaining the function of PKS gene in upland cotton, Arabidopsis thaliana (no duplicated gene was found in Arabidopsis thaliana). With qRT-PCR analysis and combination of the role of the accumulation of procyanidins in brown cotton fiber, we concluded that the five brown PKS genes are candidate genes involving in brown cotton fiber pigment synthesis. These results play an important role in the further study of brown cotton PKS genes.


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