[Experimental] List of manuscripts available for review volunteers
2 manuscripts available for review volunteers
March 10, 2017
Insecticidal crystal (Cry) proteins produced by the bacterium Bacillus thuringiensis (Bt) target cells in the midgut epithelium of susceptible larvae. While the mode of action of Cry toxins has been extensively investigated, the midgut response to Cry intoxication and its regulation are not well characterized. In this work, we report the secreted proteome (secretome) of primary mature midgut cell cultures from Heliothis virescens larvae after exposure to Cry1Ac toxin compared to control buffer treatment. Biological activity of the Cry1Ac-induced secretome was monitored as higher proliferation and differentiation and an overall reduction in total cell mortality over time in primary H. virescens midgut stem cell cultures when compared to treatment with control buffer secretome. Differential proteomics identified 4 proteins with significant differences in abundance comparing Cry1Ac-treated and control secretomes. The most significant difference detected in the Cry1Ac secretome was an arylphorin protein not detected in the control secretome. Feeding of purified arylphorin to H. virescens larvae resulted in midgut hyperplasia and significantly reduced susceptibility to Cry1Ac toxin compared to controls. These data identify arylphorin as a protein with a putative relevant role in the midgut regeneration process in response to Cry1Ac intoxication.
February 28, 2017
Background. Litchi stink-bug, Tessaratoma papillosa Drury (Hemiptera: Tessaratomidae) is a major pest on litchi and longan in Southern China. It is urgent to develop valid biological agent for control the pest and improve IPM strategy on orchard farming. Entomopathogenic fungi was regarded as avital ecological factor pressing pest populations in field, however, there were fewer searches conducted on entomopathogenic fungi against litchi stink-bug. Methods & Results. In this study, two pathogenic fungus were isolated from the adult diseased T. papillosa by normal methods and rDNA-ITS homogeneous analysis, they are identified as Paecilomyces lilacinus and Beauveria bassiana. Laboratory tests showed that the two entomopathogenic fungi both had a good lethal effect to young nymph and old nymph of T. papillosa. The toxicity determination showed that the LC50 value for Beauveria bassiana was higher than P. lilacinus. Conclusion.These pathogenic fungus did not have the risk of pollution or residue, and they can be a alternative option for integrated pest management approache.


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