[Experimental] List of manuscripts available for review volunteers
2 manuscripts available for review volunteers
September 15, 2017
Plant defensins are known for different biological functions such as insect resistance, antibacterial, antifungal and antiviral activities. The role of plant defensins against weevils is based on inhibitor of alpha-amylase activity in weevil gut, therefore, weevils can not digest starch and then die. The low content of Vigna radiata plant defensin 1 (VrPDF1) in mung bean seeds is promted researches to increase the VrPDF1content, in which leads to enhance alpha-amylase inhibition and improve bruchid resistance in mung bean. This article presents the results of overexpression of VrPDF1 gene in transgenic mung bean plants. VrPDF1 gene was successfully transferred into DX22 mung bean cultivar and expressed in T1 generation transgenic mung bean seeds. The extract containing recombinant VrPDF1 protein inhibited alpha-amylase of weevil larvae in its larval stage. The performance of alpha-amylase inhibitor of DX1-3 and DX1-7 transgenic mung bean lines increased by 166.40% and 178.19% respectively, in comparison with non-transgenic plants. The enhancement of alpha-amylase inhibitor ability of rVrPDF1 extracted from transgenic plants is scientifically fundamental to confirm the effectiveness of the application of gene technology in enhancement the ability of mung bean weevil resistance in particular and grain crops in general revenue.
August 2, 2017
We describe the \textsc{Coefficient-Flow} algorithm for calculating the bounding chain of an $(n-1)$--boundary on an $n$--manifold-like simplicial complex $S$. We prove its correctness and show that it has a computational time complexity of $O(|S^{(n-1)}|)$ (where $S^{(n-1)}$ is the set of $(n-1)$--faces of $S$). We estimate the big-$O$ coefficient which depends on the dimension of $S$ and the implementation. We present an implementation, experimentally evaluate the complexity of our algorithm, and compare its performance with that of solving the underlying linear system.


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