I read the paper, and it looks like you only compared one alpha diversity metric (species observed) between dada/unoise/deblur. I think it's a bit misleading to title a section "alpha diversity metrics" (plural) when only one is compared.

Also, since weighted beta div metrics were very similar (sup figs 6-8), I'd think that weighted alpha div metrics like shannon or weighted PD would be ro...

Hi, I have a question regarding the use of UNOISE3 in this article. On lines 123-125: Finally, the FASTA containing unique sequences was used as input into USEARCH’s unoise3 (Edgar, 2016)⁠ command generating a BIOM table and representative ASVs that were used in subsequent analyses. Isn't the UNOISE3 relaying on the abundances of the sequences ( https://drive5.com/usearch/manual/unoise_algo...