why after heat shock the competent cell have kept more then 2 minute

Standard procedure for transformation Therefore, the procedures that demonstrate that the mixture was kept on ice for 30 minutes after receiving a 90-second heat shock at 42 °C. The ice step comes before the heat shock, and the time in ice after the shock is 1 to 2 minutes, not 30 minutes.

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competent cell

Shahnawaz Ahmed • 0

• 12 May 2023 6:31am

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