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#1, Does endogenous SOCS3 protein come up on WB? Fig2A shows that SOCS3 is not present (or not detectable) in cells. ---If this endogenous SOCS3 is not detectable, shRNA study for SOCS3 might represent non-specific effect on MMP13 gene expression in this system.

#2, Was MMP13 protein detectable? ---If MMP13 is functional molecule for osteoblast function, MMP13 must be expressed as protein...

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