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We have been reading the journal, and we have been wondering if the detectable expression level (counts < 0) that were discarded happened at the TMM level or the TPM level.

We have tried to go over your steps one by one, but we kept getting different results from yours.

We would appreciate if you would be able to reply to our question. Thank you.

(Ps: Here’s my email: 21500364@handong.e...

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Reference gene detection
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