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I would like to upload to a repository a data set (including raw data .csv files, GIS shapefiles, and R code used for analysis) that I used in a journal article manuscript on conservation/ecology. This will allow the data to be accessed, used and cited by others....

["Open data","csv","Manuscript drafting","online repositories"]
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how to you get the primer mix, which comany? how much does it cost for the longforward and longreverse primer set synthesis ? how did you decide the at what concentration or mass DNA template should be used? it is depend on the diversity of the sample ? please...

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As scientists we are increasingly encouraged to communicate our findings to a general audience. Similarly, writing lectures requires us to design appealing presentations to engage effectively with students. And yet we are not always known for being the best communicators....

["Graphic design","graphs","figures","maps","reports","presentations","posters"]
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I’ve been intrigued by the expression “a mixed mechanism inducing a deontological imprimatur is required” but I have been unable to interpret it. The context in which it is used, too, is a bit difficult to understand for me. Is there an available end-user...

["Deontological imprimatur"]
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Nice paper, and thanks for submitting a preprint. I have a suggestion about the use of bar plots, that hide information about the data distribution. Below some suggestions (with code) on valid alternatives. This representation hides data and confounds the...

["statistics","data visualization."]
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The PDF-AddOn library for MeVisLab 2.8(.x) has been updated. See http://dx.doi.org/10.5281/zenodo.155381 for the latest version.

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Mainframe z/OS does not use the ASCII standard; it uses IBM's Extended Binary Coded Decimal Interchange Code (EBCDIC) character encoding - actually many different incompatible versions

["z\/OS","CKD","EBCDIC","ASCII","IBM"]
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If there's a list of ELNs, vs points in the final selection, I missed it.

["Data"]
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The Australian research community has been using this principle 9or one very similar) to measure its universities for several years now. Peter Timms (ptimms@usc.edu.au)

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For how many hours were the cells keep at low O2 before analyzing mitochondrial content and potential? Thanks

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i plan to use ammonium hydroxide for pre-treatmennt. may i know after lyophilisation, how long it can be stored in -80? is it soluble in PBS only? because im going to introduce this compound directly on brain.. so i would like to avoid using mixture of Naoh + sterile...

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I am an undergrad at Wake Forest working in Dr. Gloria Muday's lab, and I am trying to use peroxy orange 1 to monitor H2O2 in my experiments. This is the best article I have found as far as using PO1 in Arabidopsis and am hoping to use a very similar methods....

["Catalase"]
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Many thanks for making the images available online. I'd like to use the flowers are a control set of objects in a study with faces. I notice however that the resolution is very low. Would it be possible to obtain higher resolution images? Thanks, Marco

["Images"]
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You list three sensible causes, but it would seem to me from experience, OS internals (context switches, interrupts etc) and from theoretical consideration that I/O (input/output) is a major source of energy use See Eurosys Pathak 2012 20-65% savings possible...

["I\/O","energy use","mobile apps","measurement","optimization"]
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In many disciplines, it is common practice to put graduate students. lab technicians etc in the middle of authors' list. Wouldn't your scheme completely erase their contribution? It seems to me it favours established authors already (hence "Matthew effect"). How...

["paper credit","graduate student","lab technician","authorship"]