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Hi, I have a question regarding the use of UNOISE3 in this article. On lines 123-125: Finally, the FASTA containing unique sequences was used as input into USEARCH’s unoise3 (Edgar, 2016)⁠ command generating a BIOM table and representative ASVs that were used...

["Methods","unoise3"]
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I personally think it must come from specific to general not otherwise because is hard to understand until read the abstract. It is just a friendly opinion.

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I know coarse grained models usually use 5, 10, 20 fs/step, but i never read before an atomistic model using 20 fs as timestep. Is that correct? or a typo?

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It doesn't say in the legend (or the one for Fig. 2) From the provided branch support values (please note: boostrap resampling and PP provide branch support values, not "nodal support": one counts the number of taxon bipartitions, i.e. internodes, in the pseudoreplicate...

["ambiguous branch support","phylogenetic terminology"]
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Hi, I would like to know your protocol for amyloid beta lyophilization. I've found some people that have a lyophilizer, which will lyophilize my protein for me, but they have asked about the conditions that they should use. I haven't use lyophilizer before, so...

["Amyloid beta lyophilization"]
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Signalling typo: page 8/42, first row in Results section, "1850" instead of "1950" [sic]

["Note of the author: typo"]
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Signalling typo: page 11/42, "Subsampled" instead of "Subampled" [sic]

["Note of the author: typo"]
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2
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I appreciate the efforts to make the pipeline used in this study reproducible by providing code organized in a public repository. I do, however, not agree that it is useful to call the collection of scripts found in the repository an R-package. One of the central...

["Software packaging"]
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I am currrently analysing some fMRI data for language and visuospatial laterality. Basically, I administered a language task (verbal fluency) and a visuospatial task (visual short-term memory) during fMRI scanning. Using meta-analyses I have identified key...

["fMRI","laterality"]
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Itopsidema vancleavei is from the Upper Triassic Chinle Formation in Petrified Forest National Park, Arizona, not from the Early-Middle Triassic Moenkopi Formation. How does this geochronological extension change the details of this study? I would think barely...

["Itopsidema","biostratigraphy"]
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Question relates to the signal issues of this data matrix (see title) – all morphological data matrices struggle with incompatible split patterns — well reflected by the overall low bootstrap support in this preferred tree and the large soft polytomies in Fig....

["phylogenetic networks","signal incompatibility","ambiguous branch support","topological uncertainty"]
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Correlation is not automatically causation.

["Correlation or causation relationship between SOM and profits"]
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Soil texture can affect soil organic matter levels.

["soil organic matter levels"]
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Does it matter that there were 10x more pests if those farms yielded much more than farms with less pests?

["Insect pest levels"]
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It was published in 1986. Was noted on 10 best lists and can be downloaded as a small PDF here: http://www.davidpetersstudio.com/GIANTS-book.pdf

["giant animals"]

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