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Is there sufficient replication in this study to back up the claims made?

It appears that there is only 1 replicate per technician per kit per site. Although three extractions were pooled for each replicate, it is likely that there would have been differences in read number between those three extractions had they been sequenced. Therefore, it may be worth going back and sequencing the pooled extractions individually to determine if technician and extraction kit truly influence community analyses.

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