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Which was the PhiX proportion used during sequencing?

Interesting work. I would like to know which was the proportion of PhiX sequences used to increase the nucleotide variability (if you used it).

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Hi Luis,

This information is probably lost for this project (or at least I cannot find it readily on my computer). However, I have used PhiX in subsequent work and simply followed the recommendations given by Illumina, for example in "Preparing Libraries for Sequencing on the MiSeq" 15039740 Revision D. I assume there are updated recommendations that would be applicable to the platform you are using.

Best,

Angus

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