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What is the alginate bead size and how this could affect TGF transport?

Hello authors. A very interesting article.

I would like to known what is the average size of the alginate beads used in this studies, and whether the effects observed could be related to the fact that some chemical signals such as TGF could have difficulties to transport though this matrix, and thus MSCs in this culture system would be exposed to different differentiation media than MSCs in pellets or in 2D.

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1 Answer
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Accepted answer

Hi, the average size of Alginate beads were about 3mm on day 3 of culture and they grew to 4mm on day 21, while the pellet culture grew from 1mm on day 3 to 1.5-2mm by the end of the 3rd week. In our experiment the larger size of alginate beads compared to pellet culture did not aggravated chondrogenesis; on contrary it showed greater results. So it does not seem the size of beads interfere with TGF transport (at least in 3mm), although more studies are needed for a proper comparison between the size of beads and the outcome of chondrogenesis.

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