Figure 1 suggests that expression levels of CENPK varies among tumor types (tissue source). Given the relationship between centromere number and the ploidy of cells, did the average ploidy among the tumors vary? Could this affect the interpretation of the data?
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Assay results can reflect multiple parameters. Centromeres, and their binding proteins, are part of every chromosome - in some cases more than one per chromosome. Cancer cells are typically abnormal with excess chromosomes and/or chromosome parts. The quantity of transcripts could reflect gene dosage; protein abundance could reflect ploidy abnormalities as well as improved stability due to centromere binding. The results beg the question of how ploidy affects the results and whether other CENP proteins and their subunits would rise/fall as well.
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