TY - JOUR UR - https://doi.org/10.7287/peerj.preprints.832v1 DO - 10.7287/peerj.preprints.832v1 TI - Assessing the reproducibility of discriminant function analyses AU - Andrew,Rose L AU - Albert,Arianne YK AU - Renaut,Sebastien AU - Rennison,Diana J AU - Bock,Dan G AU - Vines,Tim DA - 2015/02/13 PY - 2015 KW - data curation KW - repeatability KW - data archiving KW - statistics AB - Data are the foundation of empirical research, yet all too often the datasets underlying published papers are unavailable, incorrect, or poorly curated. This is a serious issue, because future researchers are then unable to validate published results or reuse data to explore new ideas and hypotheses. While data files may be securely stored and accessible, they must also be accompanied by accurate labels and identifiers. To assess how often problems with metadata or data curation affect the reproducibility of published results, we attempted to reproduce Discriminant Function Analyses (DFAs) from the field of organismal biology. DFA is a commonly used statistical analysis that has changed little since its inception almost eight decades ago, and therefore provides an excellent case study to test reproducibility. Out of 100 papers we initially surveyed, fourteen were excluded because they did not present the common types of quantitative result from their DFA, used complex and unique data transformations, or gave insufficient details of their DFA. Of the remaining 86 datasets, there were 16 cases for which we were unable to confidently relate the dataset we received to the one used in the published analysis. The reasons ranged from incomprehensible or absent variable labels, the DFA being performed on an unspecified subset of the data, or incomplete data sets. We focused on reproducing three common summary statistics from DFAs: the percent variance explained, the percentage correctly assigned and the largest discriminant function coefficient. The reproducibility of the first two was high (20 of 25 and 43 of 59 datasets, respectively), whereas our success rate with the discriminant function coefficients was lower (15 of 36 datasets). When considering all three summary statistics, we were able to completely reproduce 46 (66%) of 70 datasets. While our results are encouraging, they highlight the fact that science still has some way to go before we have the carefully curated and reproducible research that the public expects. VL - 3 SP - e832v1 T2 - PeerJ PrePrints JO - PeerJ PrePrints J2 - PeerJ PrePrints SN - 2167-9843 ER -