Isolation and identification of Yersinia and Pseudomonas sp. from Australian milk and salad using 16s rDNA
- Published
- Accepted
- Subject Areas
- Food Science and Technology, Microbiology, Molecular Biology
- Keywords
- Mixed salad, Milk, Yersinia, Pseudomonas, PCR
- Copyright
- © 2015 Nawaz et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ PrePrints) and either DOI or URL of the article must be cited.
- Cite this article
- 2015. Isolation and identification of Yersinia and Pseudomonas sp. from Australian milk and salad using 16s rDNA. PeerJ PrePrints 3:e820v1 https://doi.org/10.7287/peerj.preprints.820v1
Abstract
A systematic research was conducted for the isolation and identification of undesirable microorganisms in food products using molecular approach. The result revealed the presence of Yersinia aleksiciae and Pseudomonas fluorescens strains in Australian pasteurized milk and mixed salad respectively. Y. aleksiciae grouped as lacking virulence marker and is regarded non-pathogenic. P. fluorescens has been identified as a marker of spoilage of ready-to-eat vegetable salads. This study confirms the rapidity and sensitivity of 16s rDNA analysis in identifying strains which contribute in early monitoring, accurate analysis and control of microbial risks in food products. The described methodology has special relevance in food quality control and safety.
Author Comment
This study is investigating the isolation, identification and characterization of microbial strains from milk and mixed salad available in Australian markets.
Supplemental Information
Agarose Gel of PCR Products
Agarose Gel of PCR Products
