On the complementation of Saccharomyces cerevisiae MET16 in Schizosaccharomyces pombe

1 Department of Biology, Boston College, Chestnut Hill, MA, USA
2 Department of Earth, Ocean and Atmospheric Science, Florida State University, Tallahassee, FL, USA
DOI
10.7287/peerj.preprints.768v1
Published
Accepted
Subject Areas
Cell Biology, Developmental Biology, Genetics, Microbiology
Keywords
S cerevisiae, S pombe, Homology, MET16, Yeast
Copyright
© 2014 Kelly
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ PrePrints) and either DOI or URL of the article must be cited.
Cite this article
Kelly TB. 2014. On the complementation of Saccharomyces cerevisiae MET16 in Schizosaccharomyces pombe. PeerJ PrePrints 2:e768v1

Abstract

Metabolically active pathways tend to be more highly conserved between ecologically similar relatives than other pathways for their critical role in life-functions. The developmental features of Schizosaccharomyces pombe and Saccharomyces cerevisiae may have become quite divergent, but their ecological niche is still quite similar. The functional homology between S. cerevisiae’s MET16p and S. pombe’s was investigated through plasmid insertion and sulfate assimilation assays in S. pombe. Although the S. cerevisiae:MET16p was produced, the S. pombe colonies were unable to utilize extracellular sulfate in the synthesis of Methionine.

Author Comment

Once further experiments can be conducted and higher quality results can be obtained, this submission will be submitted for review at PeerJ.