Immune response analysis
Immune sera from three mice and two negative controls were probed by ELISA against recombinant human PrP (recHuPrP). Serial dilutions were prepared for each sample. The sera from immunized mice were able to recognized recHuPrP up to the 10-6 dilution while both negative controls gave no reaction.
Peptides used for epitope mapping
Panel of the synthesized peptides from HuPrP sequence (1 to 11) and MoPrP sequence (12 to 15) used for epitope mapping.
Cell viability of mAb-treated cells
Both GT1 and ScGT1 cells were treated with the different mAbs for 5 days, refreshing the medium on the third day. Then cell viability was evaluated by MTT assay according to the procedure described in the Materials and Methods section. No statistical differences in term of cell viability were found in mAb-treated cells compared to untreated controls. For every mAb the average values from 5 wells are expressed as percentages of cell viability referred to untreated cells.