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Supplemental Information

Immune response analysis

Immune sera from three mice and two negative controls were probed by ELISA against recombinant human PrP (recHuPrP). Serial dilutions were prepared for each sample. The sera from immunized mice were able to recognized recHuPrP up to the 10-6 dilution while both negative controls gave no reaction.

DOI: 10.7287/peerj.preprints.694v1/supp-1

Peptides used for epitope mapping

Panel of the synthesized peptides from HuPrP sequence (1 to 11) and MoPrP sequence (12 to 15) used for epitope mapping.

DOI: 10.7287/peerj.preprints.694v1/supp-2

Cell viability of mAb-treated cells

Both GT1 and ScGT1 cells were treated with the different mAbs for 5 days, refreshing the medium on the third day. Then cell viability was evaluated by MTT assay according to the procedure described in the Materials and Methods section. No statistical differences in term of cell viability were found in mAb-treated cells compared to untreated controls. For every mAb the average values from 5 wells are expressed as percentages of cell viability referred to untreated cells.

DOI: 10.7287/peerj.preprints.694v1/supp-3

Additional Information

Competing Interests

The authors declare they have no competing interests.

Author Contributions

Alessandro Didonna conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Anja Colja Venturini conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Katrina Hartman performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Tanja Vranac performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Vladka Curin Serbec conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Giuseppe Legname conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Approval for research involving human material has been obtained from the Slovenian National Medical Ethics Committee with decision dated January 15, 2008. Post mortem brain tissue of a patient who was clinically suspected for CJD was analyzed by immunohistochemistry without patient's consent because such analysis is obligatory by a ministerial decree in purpose of TSE surveillance (Official Gazette of the Republic of Slovenia, 2/2001). Human brain samples for immunohistochemistry were obtained from the Institute of Pathology, Faculty of Medicine, University of Ljubljana, Slovenia.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

All experiments involving animals were performed in accordance with European regulations [European Community Council Directive, November 24, 1986 (86/609/EEC)]. Experimental procedures were notified to and approved by the Italian Ministry of Health, Directorate General for Animal Health (notification of 17 Sept. 2012). All experiments were approved by the local authority veterinary service and by SISSA Ethics Committee. All reasonable efforts were made to ameliorate suffering. All mice were obtained from the European Mutant Mouse Archive.

Funding

This study was supported by the European Community’s Seventh Framework Programme (FP7/2007–2013) under grant agreement n° 222887—the PRIORITY project to GL and by the Slovenian Research Agency with Research Programme P4―0176, Research Project L3-0206 and with PhD grant to ACV. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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