Accessions included in study
Accessions of Zea mays ssp. mexicana (RIMME) and Zea mays ssp. parviglumis (RIMPA) sampled. RIHY is a Zea mays ssp. parviglumis and Zea mays ssp. mays hybrid
Hopscotch frequency in maize
Hopscotch frequency in sampled Zea mays ssp. mays (RIMMA).
Depiction of tb1 region sequenced
Representation of the upstream regulatory region of tb1, showing the tb1 coding region (green) and the Hopscotch insertion (red). Arrows show the location of primer sets; in black, primers used for amplification and sequencing (Region 1; within the 5' UTR, and Region 2; 66,169 bp upstream from the tb1 ORF); in blue, primers used to genotype the Hopscotch insertion.
Neighbor-joining tree based on tb1 sequences
Neighbor-joining tree of the sequenced region in the 5' UTR (right; Region 1) and the 66,169 bp upstream region (left; Region 2) of tb1. Individuals with genotype data are colored: Homozygous for the teosinte (no Hopscotch) allele (red), homozygous for the maize (Hopscotch) allele (blue), heterozygotes (purple). TILs (teosinte inbred lines) are colored in green, with stars indicating the 3 TILs known to have the Hopscotch insertion. Black indicates individuals not genotyped for the Hopscotch insertion. EjuA refers to individuals from population Ejutla A, EjuB from Ejutla B, SLO from San Lorenzo, and MSA from La Mesa. Remaining individuals are lines of maize (Zea mays ssp. mays)