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Supplemental Information

Accessions included in study

Accessions of Zea mays ssp. mexicana (RIMME) and Zea mays ssp. parviglumis (RIMPA) sampled. RIHY is a Zea mays ssp. parviglumis and Zea mays ssp. mays hybrid

DOI: 10.7287/peerj.preprints.685v1/supp-1

Hopscotch frequency in maize

Hopscotch frequency in sampled Zea mays ssp. mays (RIMMA).

DOI: 10.7287/peerj.preprints.685v1/supp-2

Depiction of tb1 region sequenced

Representation of the upstream regulatory region of tb1, showing the tb1 coding region (green) and the Hopscotch insertion (red). Arrows show the location of primer sets; in black, primers used for amplification and sequencing (Region 1; within the 5' UTR, and Region 2; 66,169 bp upstream from the tb1 ORF); in blue, primers used to genotype the Hopscotch insertion.

DOI: 10.7287/peerj.preprints.685v1/supp-3

Neighbor-joining tree based on tb1 sequences

Neighbor-joining tree of the sequenced region in the 5' UTR (right; Region 1) and the 66,169 bp upstream region (left; Region 2) of tb1. Individuals with genotype data are colored: Homozygous for the teosinte (no Hopscotch) allele (red), homozygous for the maize (Hopscotch) allele (blue), heterozygotes (purple). TILs (teosinte inbred lines) are colored in green, with stars indicating the 3 TILs known to have the Hopscotch insertion. Black indicates individuals not genotyped for the Hopscotch insertion. EjuA refers to individuals from population Ejutla A, EjuB from Ejutla B, SLO from San Lorenzo, and MSA from La Mesa. Remaining individuals are lines of maize (Zea mays ssp. mays)

DOI: 10.7287/peerj.preprints.685v1/supp-4

Gel image of PCR products

Agarose gel image of amplification products using our primer sets. Genotypes are indicated at the top of the gel.

DOI: 10.7287/peerj.preprints.685v1/supp-5

Additional Information

Competing Interests

Jeffrey Ross-Ibarra is an Academic Editor for PeerJ.

Author Contributions

Laura Vann performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Thomas Kono performed the experiments, analyzed the data, reviewed drafts of the paper.

Tanja Pyhäjärvi analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Matthew B. Hufford conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Jeffrey Ross-Ibarra conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

Figshare <http://dx.doi.org/10.6084/m9.figshare.776926>, <http://dx.doi.org/10.6084/m9.figshare.779707>. Sanger sequencing data will be uploaded to Figshare prior to publication

Data Deposition

The following information was supplied regarding the deposition of related data:

Figshare: <http://dx.doi.org/10.6084/m9.figshare.1166630>, <http://dx.doi.org/10.6084/m9.figshare.776926>, <http://dx.doi.org/10.6084/m9.figshare.1165577>.

Funding

Funding was provided by the Department of Plant Sciences at UC Davis for graduate student research funding to LEV and for research funds supporting the project, and by UC Mexus for a postdoctoral scholar grant to MBH and JR-I. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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