Fast Track DNA Analysis Suite for human identification
1
Technical, Diamond Light Source, Didcot, England
2
Department of Biology, University of Reading, Reading, England
3
Department of Life Sciences, University of Reading, Reading, England
4
Department of Chemistry, University of Hull, Hull, England
- Published
- Accepted
- Subject Areas
- Biochemistry, Bioengineering, Genetics, Genomics
- Keywords
- Genetic fingerprinting, Portable DNA analyser, Genetic profiling, Forensics
- Copyright
- © 2013 Docker et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Cite this article
- 2013. Fast Track DNA Analysis Suite for human identification. PeerJ PrePrints 1:e6v1 https://doi.org/10.7287/peerj.preprints.6v1
Abstract
This paper details the development of a portable ‘Lab on chip’ DNA analyser that was developed to facilitate rapid analysis of DNA samples for ‘at scene of crime’ and in custody suite situations where human identification is required rapidly. This system was proven to work with human DNA for 3 loci, namely VWA, D21 and D18 taken from raw sample through PCR separation to detection within 90miniutes. Once the sample was loaded onto the microfluidic chip which in turn was loaded into the instrument no further human interaction took place. This paper details the approach to the biochemistry, hardware before going on to give results proving the proof of principle and then the authors’ conclusions.