Understanding the role of Oryza sativa OsPILS (PIN Like) genes in auxin signaling
- Published
- Accepted
- Subject Areas
- Bioinformatics, Developmental Biology, Genomics, Molecular Biology, Plant Science
- Keywords
- AtPIN, OsPIN, OsPILS, AtPILS
- Copyright
- © 2014 Mohanta et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ PrePrints) and either DOI or URL of the article must be cited.
- Cite this article
- 2014. Understanding the role of Oryza sativa OsPILS (PIN Like) genes in auxin signaling. PeerJ PrePrints 2:e586v1 https://doi.org/10.7287/peerj.preprints.586v1
Abstract
The phytohormone auxin is one of the most important signaling molecule that undergo accumulation or depletion in temporal or spatial manner due to wide arrays of changes in developmental or stress programme. Proper distribution, maintenance and homeostasis of auxin molecules across the plant system is one of the most important phenomena. The distribution and homeostasis of auxin is maintained by auxin transport system across the plant. Auxin transportation is carried out by auxin transporter family proteins, popularly known as auxin efflux carriers (PINs). Besides, auxin efflux carrier family proteins, a sub-family of auxin efflux carriers (OsPILS) being identified from Oryza sativa and reported here. Oryza sativa encodes for seven putative sub-cellularly localized transmembrane PILS proteins. Differential expression of OsPILS genes are found to be regulated by auxin and cytokinin dependent manner. In auxin treated plants, all OsPILS genes are up-regulated in leaf tissues and down regulated in root tissues during third week time point. In cytokinin treated plants, maximum of OsPILS genes were up-regulated both in leafs and roots tissues during third week time period. Regulation of gene expression of OsPILS genes by auxin and cytokinin during third week time period reflects its important roles during plant growth and development.
Author Comment
This is a submission to PeerJ for review.
Supplemental Information
Supplementary Figure
Figure showing multiple sequence alignment of OsPIN, AtPIN, AtPILS and OsPILS genes. Sequence alignment show that although PINs and OsPILS genes are auxin efflux carrier, their amino acid sequences are quite distinct from each other. Alignment of PILS genes are starts at the end of the PIN genes. As PILS genes are evolutionarily conserved from algae to higher eukaryotes, PINs and PILS genes are supposed to be existed as single gene and got diversified during the time of evolution as complexity of organism get increased.