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Additional Information

Competing Interests

Arti Ahluwalia is an Academic Editor for PeerJ.

Author Contributions

Giorgio Mattei conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Irene Cristiani performed the experiments, wrote the paper.

Chiara Magliaro conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Arti Ahluwalia conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Experiments were conducted in conformity with the European Communities Council Directive of 24 November 1986 (86/609/EEC and 2010/63/UE) and in agreement with the Italian DM26/14. Experiments were approved by the Italian Ministry of Health and Ethic Committee of the University of Pisa.

Data Deposition

The following information was supplied regarding the deposition of related data:

The software Stegun can be downloaded at http://www.centropiaggio.unipi.it/software

Funding

The work leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement n° 304961 (ReLiver). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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