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Supplemental Information

The relative expression of miR-142 and miR-21 in CCR6 + Tregs

CCR6 + Tregsand CCR6 - Tregs were purified from splenocytes in Balb/c mice by FACSsorting. The relative expression of miR-142 and miR-21 in CCR6 + Tregscells was determined by Realtime PCR assay.

DOI: 10.7287/peerj.preprints.471v2/supp-1

The expression of putative targets of miR-142a in CCR6+Tregs

CCR6 + Tregsand CCR6 - Tregs were purified from splenocytes in Balb/c mice by FACSsorting. The global expression of genes in cells was analyzed by microarrayarray and then the expression of putative target genes was listed (CCR6 - Tregs; CCR6 + Tregs)

DOI: 10.7287/peerj.preprints.471v2/supp-2

Additional Information

Competing Interests

The authors declare they have no competing interests.

Author Contributions

Juanjuan Zhao analyzed the data, wrote the paper, reviewed drafts of the paper.

Yongju Li performed the experiments, analyzed the data, prepared figures and/or tables, reviewed drafts of the paper.

Yan Hu performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, reviewed drafts of the paper.

Chao Chen analyzed the data, reviewed drafts of the paper.

Ya Zhou performed the experiments, contributed reagents/materials/analysis tools, prepared figures and/or tables, reviewed drafts of the paper.

Yijin Tao performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Mengmeng Guo performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Nalin Qin performed the experiments, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Lin Xu conceived and designed the experiments, performed the experiments, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Zunyi Medical College Laboratory Animal Care and Use Committee (No.20130108)

Microarray Data Deposition

The following information was supplied regarding the deposition of microarray data:

Data accessible at NCBI GEO database (Xu L et al., 2014), reference GSE60041

Funding

This work was supported by National Natural Science foundation of China (81260398, 31370918), Program for New Century Excellent Talents in University, Ministry of Education of China (NCET-12-0661), International Cooperation Foundation of Guizhou Province (2010-7031), Specific Foundation for the Scientific Educational Talent of President of Guizhou Province (09C457), Project of Guizhou Provincial Department of Science and Technology (2009C491) and Zunyi Medical College Start-up Fund (2008F-329). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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