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Supplemental Information

Distribution of observed SNPs in the TcSMO-like genes of T. cruzi.

Based on the prediction of trans-membrane spanning domains (see TMHMM probability plot at the bottom), we created two alternative representations, following Sperling, Ternes, Zank et al (2003). The distribution of synonymous and non- synonymous SNPs is shown according to these models. The representations differ in the presence/absence of the second (non-predicted) trans-membrane domain. In these two representations the location of the 3rd histidine box always lies on the opposite side of the membrane. Both topologies may be wrong and an in-depth study (similar to the one performed by Diaz, Mansilla, Vila et al., 2002) may be required to establish the correct topology of these proteins.

DOI: 10.7287/peerj.preprints.44v1/supp-1

Reciprocal BLASTP searches between fungal and kinetoplastid ERG25/ERG3 homologs.

The file contains a summary of BLASTP searches against fungal and kinetoplastid protein databases. BLAST searches using the yeast ERG3/ERG25 protein sequences as query, were run at the TriTrypDB BLAST server against a database of Kinetoplastid proteomes from reference and draft genomes. BLAST searches using a number of putative T. cruzi orthologs of these yeast genes were run at the SGD Fungal BLAST Server, against a database containing a selection of fungal genomes. Each BLASTP search is shown in a separate tab in the Excel workbook.

DOI: 10.7287/peerj.preprints.44v1/supp-2

List of nucleotide changes (SNPs, fixed differences) identified for each gene analyzed.

The excel file contains one spreadsheet per gene with information on the location of each SNP relative to the start codon, the PolyPhred score for the SNP, and the character state of the SNP in each strain/lineage.

DOI: 10.7287/peerj.preprints.44v1/supp-3

List of oligonucleotide primers and amplification products analyzed in this study.

The table lists the oligonucleotide primers used to amplify each PCR product, the corresponding product size, and the number of products per gene/locus.

DOI: 10.7287/peerj.preprints.44v1/supp-4

Additional Information

Competing Interests

The authors declare that no competing interests exist.

Author Contributions

Raul O Cosentino conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper.

Patricio Diosque analyzed the data, contributed reagents/materials/analysis tools, wrote the paper.

Fernán Agüero conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper.

Grant Disclosures

The following grant information was disclosed by the authors:

Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT, Argentina), PICT-2010-1479.

Special Programme for Research and Training in Tropical Diseases (WHO / UNDP / UNICEF / World Bank), A50830.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

GenBank JN050313-JN050853, KF290395-KF290460

Data Deposition

The following information was supplied regarding the deposition of related data:

Figshare, http://dx.doi.org/10.6084/m9.figshare.747608

Funding

This work was supported by grants from the National Agency for the Promotion of Science and Technology (ANPCyT, Argentina), the National Research Council of Argentina, and the Special Programme for Research and Training in Tropical Diseases (WHO / UNDP / UNICEF / World Bank). RC is now a fellow of the National Research Council of Argentina (CONICET) and was supported during this work by a fellowship from the University of San Martín. FA and PD are members of the Research Career of CONICET. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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