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Supplemental Information

Supplementary File 1

R scripts and data.

DOI: 10.7287/peerj.preprints.420v1/supp-1

Supplementary File 2

Sequence data for 21 Plectostoma species and 4 outgroup taxa in nexus format for MrBayes analysis.

DOI: 10.7287/peerj.preprints.420v1/supp-2

Supplementary File 3

Character matrix.

DOI: 10.7287/peerj.preprints.420v1/supp-3

Supplementary File 4

Aperture ontogeny profiles for 11 Plectostoma species raw data.

DOI: 10.7287/peerj.preprints.420v1/supp-4

Supplementary File 5

Aperture ontogeny profiles for 11 Plectostoma species, in which each was labelled by respective shell shape.

DOI: 10.7287/peerj.preprints.420v1/supp-5

Supplementary File 6

Phylogenetic tree inferred by maximum likelihood analysis

DOI: 10.7287/peerj.preprints.420v1/supp-6

Supplementary File 7

Ancestral state reconstructions for shell shape and size, and rib form, as derived by the maximum parsimony method.

DOI: 10.7287/peerj.preprints.420v1/supp-7

Additional Information

Competing Interests

Thor-Seng Liew and Menno Schilthuizen are employees of the Naturalis Biodiversity Center. The authors have declared that no competing interests exist.

Author Contributions

Thor-Seng Liew conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables.

Menno Schilthuizen contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

The permissions for collecting specimens in Malaysia were given to LTS by the Economic Planning Unit, Prime Minister’s Department (UPE: 40/200/19/2524), State Planning Unit, Chief Minister’s Department, Sarawak ((47) UPN/S/G1/I/10.1 Vol.27), Forest Department Sarawak (Research Permit NPW.907.4.4(V)-19; Park Permit No. 07/2010; Export Permit No. 09003).

Grant Disclosures

The following grant information was disclosed by the authors:

This study is funded under project 819.01.012 of the Research Council for Earth and Life Sciences (ALW-NWO). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

Genbank:

KC420367, KC420316, KC420413, KC420271, KC420400, KC420353, KC420451, KC420304, KC250938, KC250963, KC250913, KC250872, KC250946, KC250971, KC250921, KC250879, KC250929, KC250954, KC250903, KC250862, KC420366, KC420315, KC420412, KC420270, KC420380, KC420332, KC420431, KC420372, KC420323, KC420421, KC420279, KC250924, KC250949, KC250898, KC250857, KC420370, KC420321, KC420419, KC420277, KC420392, KC420345, KC420443, KC420297, KC250934, KC250959, KC250909, KC250868, KC250926, KC250951, KC250900, KC250859, KC250936, KC250961, KC250911, KC250870, KC250932, KC250957, KC250906, KC250865, KC250925, KC250950, KC250899, KC250858, KC420374, KC420325, KC420423, KC420281, KC250941, KC250966, KC250916, KC250875

Funding

This study is funded under project 819.01.012 of the Research Council for Earth and Life Sciences (ALW-NWO). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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