Strategies for resolving skeleton junctions
(A) A minimal skeleton. (B) Skan’s classification of pixels into endpoints, paths, and junctions based on the number of neighbours (1, 2, and 3 or more, respectively). (C) Identical classification in Fiji’s Analyze Skeletons. (D) Skeleton measurement when junctions are assigned an implicit “extent”. (E) Skeleton measurement when all adjacent junction pixels are replaced by their centroid (our default strategy). (F) Skeleton measurement used in Fiji’s Analyze skeletons (mid-2017 version).